超高效液相色谱-串联质谱法测定人血浆中尼洛替尼的研究

发布时间：2018-12-10 09:48

【摘要】：目的建立超高效液相色谱-串联质谱(UPLC-MS/MS)法测定血浆中尼洛替尼浓度方法。方法以卡马西平为内标,用蛋白沉淀前处理方法。色谱柱为ACQUITY UPLC BEH C18(100 mm×2.1 mm,1.7μm),流动相为乙腈-0.1%甲酸水溶液(35∶65),流速为0.4 m L·min-1,正离子模式多反应监测(MRM)扫描分析,并考察专属性、标准曲线与定量下限、精密度与回收率、基质效应和稳定性。结果血浆中尼洛替尼线性范围为0.1~20.0μg·m L-1(r=0.998),定量下限为0.1μg·m L-1,提取回收率在95.11%~101.32%,日内、日间精密度相对标准偏差(RSD)均小于15%。结论该法操作简便快速,特异性强,灵敏度高,可用于尼洛替尼的治疗药物浓度监测。
[Abstract]:Objective to establish a high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of nilatinib in plasma. Methods carbamazepine was used as internal standard and protein precipitation was used. The chromatographic column consisted of ACQUITY UPLC BEH C18 (100 mm 脳 2.1 mm,1.7 渭 m),) mobile phase was acetonitrile-0.1% formic acid aqueous solution (35:65), and the flow rate was 0.4 mL min-1, positive ion mode multi-reaction monitoring (MRM) scanning analysis. The specificity, standard curve and lower limit of quantification, precision and recovery, matrix effect and stability were investigated. Results the linear range of nilotinib in plasma was 0.1 渭 g / mL ~ (-1) (r = 0.998), and the lower limit of quantification was 0.1 渭 g / mL ~ (-1). The recovery was 95.11 渭 g / mL ~ (-1) and the recovery rate was 101.32 渭 g / m ~ (-1). The relative standard deviation (RSD) of daytime precision was less than 15. Conclusion this method is simple, rapid, specific and sensitive. It can be used to monitor the concentration of nirotini.
【作者单位】：苏州大学附属第一医院药学部;
【分类号】：R96;O657.63

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