舍曲林诱导Kasumi-1细胞凋亡及其作用机制的实验研究

发布时间：2018-12-17 00:02

【摘要】：目的:探讨精神类药物舍曲林对人急性髓系白血病细胞株Kasumi-1的生物学效应。方法:将不同浓度舍曲林作用于Kasumi-1细胞不同时间,通过细胞计数、细胞形态学检查、流式细胞术和Western blot分别检测舍曲林处理前后Kasumi-1细胞增殖和凋亡情况、细胞周期分布以及相关蛋白的改变。结果:舍曲林能够抑制Kasumi-1细胞增殖、诱导细胞凋亡;15和20μmol/L舍曲林处理Kasumi-1细胞24 h后,生长抑制率分别达到(19.00±7.37)%和(47.90±11.19)%;流式细胞术检测结果显示,与空白对照组相比,15和20μmol/L舍曲林处理Kasumi-1细胞24 h后,Annexin V阳性细胞百分比从(9.71±2.12)%分别上升至(20.54±2.52)%和(45.37±7.88)%(P0.01),且细胞被明显阻滞在G0/G1期和G2/M期;此外,舍曲林还可以下调细胞内翻译控制肿瘤蛋白(translationally controlled tumor protein,TCTP)。结论:舍曲林能够显著诱导Kasumi-1细胞发生凋亡,其作用机制可能与细胞内TCTP蛋白的下调相关。
[Abstract]:Objective: to investigate the biological effects of sertraline on human acute myeloid leukemia cell line Kasumi-1. Methods: Kasumi-1 cells were treated with Sertraline at different concentrations for different time. Cell count, cell morphology, flow cytometry and Western blot were used to detect the proliferation and apoptosis of Kasumi-1 cells before and after sertraline treatment. Cell cycle distribution and changes in related proteins. Results: Sertraline inhibited the proliferation of Kasumi-1 cells and induced apoptosis, and the growth inhibition rates of Kasumi-1 cells treated with sertraline for 24 h were (19.00 卤7.37)% and (47.90 卤11.19)%, respectively. The results of flow cytometry showed that Kasumi-1 cells were treated with 15 and 20 渭 mol/L sertraline for 24 h, compared with the control group. The percentage of Annexin V positive cells increased from (9.71 卤2.12)% to (20.54 卤2.52)% and (45.37 卤7.88)%, respectively. In addition, sertraline can also down-regulate intracellular translation control tumor protein (translationally controlled tumor protein,TCTP). Conclusion: Sertraline can induce apoptosis in Kasumi-1 cells, and its mechanism may be related to the down-regulation of TCTP protein.
【作者单位】：上海交通大学医学院附属瑞金医院检验系上海血液学研究所医学基因组学国家重点实验室;
【基金】：国家自然科学基金(81170508,81470317) 上海市领军人才"国家队"专项资金
【分类号】：R96

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