脑脉泰对ApoE基因敲除小鼠动脉粥样硬化斑块稳定性的影响

发布时间：2018-12-18 06:39

【摘要】：目的探讨脑脉泰对ApoE基因敲除小鼠动脉粥样硬化斑块稳定性影响及其机制。方法采用ApoE基因敲除小鼠制备动脉粥样硬化模型,具有相同遗传背景的C57BL/6J小鼠作为正常对照组,分别给予不同剂量的脑脉泰和阿托伐他汀。高脂饲料喂养16 w后HE染色法观察主动脉根部斑块大小,免疫组化法检测动脉斑块MMP-2、MMP-9和巨噬细胞CD68的表达。结果与正常组相比,模型组斑块明显增大,阿托伐他汀组和脑脉泰组斑块面积显著减小且阿托伐他汀组和脑脉泰组斑块大小无显著性差异。免疫组化结果显示与模型组相比,给予脑脉泰药物后斑块处MMP-9、MMP-2和CD68表达显著性下降(P0.05),但与阿托伐他汀组相比无显著性差异。结论脑脉泰通过降低MMP-9、MMP-2和CD68的表达减少不稳定斑块的破损。
[Abstract]:Objective to investigate the effect and mechanism of Naomaitai on the stability of atherosclerotic plaque in ApoE knockout mice. Methods ApoE knockout mice were used to prepare atherosclerosis model. C57BL/6J mice with the same genetic background were treated with different doses of Naomaitai and Atto vastatin respectively. The size of aortic plaques was observed by HE staining and the expression of MMP-2,MMP-9 and macrophage CD68 in aortic plaques were detected by immunohistochemical method after feeding with high fat diet for 16 weeks. Results compared with the normal group, the plaque size in the model group was significantly increased, the plaque area in the Atto vastatin group and the Naomaitai group was significantly decreased, but there was no significant difference in the plaque size between the Atto vastatin group and the Naomaitai group. Immunohistochemical results showed that the expression of MMP-9,MMP-2 and CD68 in plaques decreased significantly after Naomaitai administration (P0.05), but there was no significant difference compared with Atto vastatin group. Conclusion Naomaitai can reduce the damage of unstable plaque by decreasing the expression of MMP-9,MMP-2 and CD68.
【作者单位】：吉林大学基础医学院药理系;吉林大学白求恩第一医院神经内科;
【基金】：国家中药保护品种改进提高示范项目课题
【分类号】：R965

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