达肝素钠和肝素钠对小鼠毒性的比较

发布时间：2018-12-19 19:03

【摘要】：目的比较达肝素钠和肝素钠对小鼠毒性的差异。方法采用半数致死量(LD_(50))法测定两药对小鼠的急性毒性,达肝素钠设6个剂量组,最低剂量为2.54×10~5U·kg~(-1);肝素钠设5个剂量组,最低剂量为2.05×10~5U·kg~(-1);剂量间距均为1.25;达肝素钠和肝素钠干预L-929细胞48 h,CCK-8法检测细胞存活率并计算半数抑制浓度(IC_(50)),达肝素钠设7个浓度,最低浓度为0.78×10~5U·L~(-1),肝素钠设6个浓度,最低浓度为0.39×10~5U·L~(-1);剂量间距均为2;采用碘化丙啶染色用流式细胞仪对L-929细胞进行细胞周期分析。结果达肝素钠和肝素钠对小鼠的LD_(50)分别为4.44×10~5和3.68×10~5U·kg~(-1);对L-929细胞的IC_(50)分别为1.42×10~6和3.54×10~5U·L~(-1)。L-929细胞经达肝素钠处理后,G_2/M期细胞百分率增加137.6%(P0.01);经肝素钠处理后,G_0/G_1期细胞百分率增加33.8%(P0.01)。结论达肝素钠对小鼠及L-929细胞的毒性均小于肝素钠,达肝素钠和肝素钠对细胞周期的影响分别阻滞在G_2/M期和G_0/G_1期。
[Abstract]:Objective to compare the toxicity of heparin sodium and heparin sodium in mice. Methods half lethal dose (LD_ (50) method was used to determine the acute toxicity of the two drugs in mice. Heparin sodium was divided into 6 dose groups with a minimum dose of 2.54 脳 10 ~ 5U kg~ (-1). Heparin sodium was divided into 5 dose groups, the lowest dose was 2.05 脳 10 ~ (5) U kg~ (-1), the dose spacing was 1.25; The survival rate of L-929 cells was measured by CCK-8 method after intervention of heparin sodium and heparin sodium for 48 h. The median inhibitory concentration (IC_) was calculated (IC_ (7 concentrations for 50), the lowest concentration was 0.78 脳 10 5 U L ~ (-1). Heparin sodium was set at 6 concentrations, the lowest concentration was 0.39 脳 10 ~ (5) U L ~ (-1); The cell cycle of L-929 cells was analyzed by flow cytometry. Results the LD_ (50) of diheparin sodium and heparin sodium to mice were 4.44 脳 10 ~ (5) U kg~ (-1) and 3.68 脳 10 ~ (5) U kg~ (-1), respectively. The IC_ (50) of L-929 cells was 1.42 脳 10 ~ (-6) and 3.54 脳 10 ~ (5) U L ~ (-1) respectively. After treated with heparin sodium, the percentage of G2 / M phase cells increased by 137.6% (P0.01). After treated with heparin sodium, the percentage of cells in G_0/G_1 phase increased by 33.8% (P0.01). Conclusion the toxicity of dheparin sodium to mice and L-929 cells was less than that of heparin sodium. The effects of heparin sodium and heparin sodium on cell cycle were blocked in G 2 / M phase and G_0/G_1 phase, respectively.
【作者单位】：中国食品药品检定研究院药理室;中国食品药品检定研究院细胞资源保藏研究中心;贵州食品药品检验所药理室;
【分类号】：R965

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