高效液相色谱法检测丙泊酚及依托咪酯混合液化学稳定性的研究
发布时间：2018-12-26 09:43
【摘要】：目的采用高效液相色谱法(HPLC)体外模拟体内环境条件确定丙泊酚、依托咪酯混合后的新物质生成情况。方法采用十八烷基硅烷键合硅胶作为填充剂(250.0mm×4.6mm,5μm),以磷酸盐缓冲液-乙腈(调至pH7.4)作为流动相[磷酸盐缓冲液∶乙腈=35∶65(V/V)],设定柱温为30℃,流速为1.0mL/min,进样量为10μL,全波长下进行紫外光谱检测(波长190~800nm),通过对比标准品及对照品图谱确认图谱中是否有新的特征峰。结果通过与标准品图谱进行比较发现,丙泊酚、依托咪酯二者混合品在体外模拟体内环境条件下无新的特征峰出现,即未生成第3种物质。结论所建方法简便、快速、准确地检测出丙泊酚、依托咪酯混合后无新物质生成。
[Abstract]:Objective to determine the production of propofol and etomidate by (HPLC) in vitro. Methods Octadecyl silane bonded silica gel was used as filler (250.0mm 脳 4.6 mm ~ 5 渭 m),) and phosphate buffer solution and acetonitrile (pH7.4) were used as mobile phase [phosphate buffer: acetonitrile = 35:65 (V / V)]. The column temperature was set at 30 鈩，
本文编号：2391908
[Abstract]:Objective to determine the production of propofol and etomidate by (HPLC) in vitro. Methods Octadecyl silane bonded silica gel was used as filler (250.0mm 脳 4.6 mm ~ 5 渭 m),) and phosphate buffer solution and acetonitrile (pH7.4) were used as mobile phase [phosphate buffer: acetonitrile = 35:65 (V / V)]. The column temperature was set at 30 鈩，
本文编号：2391908
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