20-羟基蜕皮甾酮对2型糖尿病大鼠海马抗氧化能力的影响

发布时间：2019-01-06 19:18

【摘要】：目的为了探讨20-羟基蜕皮甾酮(20E)对2型糖尿病大鼠神经细胞的影响及相关机制。方法大鼠造模结束后,将造模成功大鼠分为模型组、二甲双胍组和20E治疗组。正常组和模型组灌服蒸馏水,二甲双胍照组和20E治疗组分别灌胃5 mg/kg盐酸二甲双胍和10 mg/kg 20E溶液。药物干预6周后,HE染色观察海马的形态学变化,real-time PCR分析海马超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和核因子кB的表达,ELISA测定脑组织脑源性神经因子和转化生长因子-β1的水平。结果与模型组相比,20E处理后海马细胞损伤程度减轻,海马中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶m RNA水平显著增加,核因子кB表达水平明显降低,脑组织脑源性神经因子含量增加。结论 20E具有减轻2型糖尿病大鼠海马细胞损伤的作用,其机制可能与提高脑组织组织抗氧化能力有关。
[Abstract]:Objective to investigate the effect of 20-hydroxyl ecdysterone (20E) on nerve cells in type 2 diabetic rats and its related mechanism. Methods the rats were divided into three groups: model group, metformin group and 20 E treatment group. The normal group and the model group were given distilled water, the metformin group and the 20E group were given 5 mg/kg metformin hydrochloride and 10 mg/kg 20E solution respectively. After 6 weeks of intervention, the morphological changes of hippocampus were observed by HE staining. The expressions of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and nuclear factor B were detected by real-time PCR. The levels of brain-derived nerve factor and transforming growth factor-尾 1 in brain tissue were measured by ELISA. Results compared with the model group, the damage degree of hippocampal cells was reduced after 20E treatment, and the levels of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase m RNA in hippocampus were significantly increased. The expression of nuclear factor B was significantly decreased and the content of brain-derived neurofactor was increased. Conclusion 20E can attenuate the damage of hippocampal cells in type 2 diabetic rats, and its mechanism may be related to the increase of antioxidant capacity of brain tissue.
【作者单位】：南阳医学高等专科学校基础医学部;
【基金】：河南省重点科技攻关项目(122102310103)
【分类号】：R965

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