NMR法测定A,C,Y和W135群脑膜炎球菌荚膜多糖氧乙酰基含量

发布时间：2019-01-09 14:50

【摘要】：目的:采用核磁共振(nuclear magnetic resonance,NMR)技术检测A,C,Y和W135群脑膜炎球菌荚膜多糖(groups A,C,Y,W135 Neisseria meningococcal capsular polysaccharides,GAMP,GCMP,GYMP,GWMP)氧乙酰基含量。方法:对A,C,Y和W135群脑膜炎球菌荚膜多糖样品进行溶解,离心,转移等处理步骤,采用核磁共振谱仪检测,并对检测参数D1,NS和计算标准进行优化使其满足定量检测要求。结果:确定的检测条件:D1值GAMP为18,GCMP,GYMP和GWMP为20;NS值均为128次,计算标准GAMP采用H-3和H-4与H1积分面积之比,GCMP采用H-7和H-8与H-3e积分面积之比,GYMP和GWMP采用H-7和H-9与H-3e积分面积之比。结论:NMR法简便、快速、准确,可用于检测A,C,Y和W135群脑膜炎球菌荚膜多糖氧乙酰基含量。
[Abstract]:Objective: to detect the content of oxygen acetyl groups of groups Con Y and W135 groups of meningococcal capsule polysaccharides (groups) by nuclear magnetic resonance (nuclear magnetic resonance,NMR). Methods: the capsular polysaccharides of Acanthoxy and W135 groups of meningococcal cocci were dissolved, centrifuged, transferred and detected by nuclear magnetic resonance spectroscopy (NMR). NS and the calculation standard are optimized to meet the requirements of quantitative detection. Results: the determined detection conditions were as follows: D1 GAMP was 18 GCMP GYMP and GWMP was 20; The ratio of H-3 and H-4 to H1 integral area in standard GAMP, H-7 and H-8 to H-3e integral area in GCMP and H-7 and H-9 to H-3e integral area in GYMP and GWMP were calculated. Conclusion: the method of NMR is simple, rapid and accurate. It can be used for the determination of the content of polysaccharide oxyacetyl in capsule of Agnin Y and W135 groups of meningococci.
【作者单位】：兰州生物制品研究所有限责任公司;
【基金】：国家“重大新药创制”科技重大专项资助项目(2013ZX09402-302-215) 甘肃省科技重大专项资助项目(0801NKDA003) 甘肃省“十二五”陇药产业发展规划项目
【分类号】：R927.2

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