吲哚菁绿金纳米笼的制备及体外抗肿瘤活性研究

发布时间：2019-01-23 14:28

【摘要】：目的:制备吲哚菁绿金纳米笼(ICG/Biotin-PEG-AuNC-PCM),研究其体外抗肿瘤活性。方法:使用多元醇还原法制备银纳米立方体(Ag NC)作为模板,通过离子置换反应制备金纳米笼(AuNC)。对AuNC形貌、粒径及近红外(NIR)光热性质进行表征。使用有机溶剂挥发法制备装载ICG、1-十四醇(PCM)、表面修饰生物素聚乙二醇巯基(Biotin-PEG-SH)的ICG/Biotin-PEG-AuNC-PCM,检测其粒径、多分散系数(PDI)、载药量,考察其在37、40℃下180 min内的体外累积释放度(Q)。以耐阿霉素人乳腺癌细胞(MCF-7/ADR细胞)为研究对象,采用MTT法考察ICG、ICG/PEG-AuNC-PCM、ICG/Biotin-PEG-AuNC-PCM的抗肿瘤活性,计算半数抑制浓度(IC50)。结果:AuNC为立方体,粒径为60 nm左右,具有良好的光热性质。ICG/Biotin-PEG-AuNCPCM的粒径为(105±2.8)nm,PDI为0.261±0.02(n=3);载药量为1.34×108g/mol AuNC;37℃下Q180 min约为10%,40℃下Q20 min约为80%。ICG、ICG/PEG-AuNC-PCM、ICG/Biotin-PEG-AuNC-PCM对MCF-7/ADR细胞的IC50分别为95.2、29.3、16.1μg/m L。结论:成功制得ICG/Biotin-PEG-AuNC-PCM,其对MCF-7/ADR细胞的抗肿瘤活性强于ICG。
[Abstract]:Aim: to prepare indocyanine green gold nanoparticles (ICG/Biotin-PEG-AuNC-PCM) and to study its anti-tumor activity in vitro. Methods: silver nanocrystalline (Ag NC) was prepared by polyol reduction method, and gold nanocage (AuNC). Was prepared by ion replacement reaction. AuNC morphology, particle size and near infrared (NIR) photothermal properties were characterized. ICG/Biotin-PEG-AuNC-PCM, loaded with ICG,1- tetradecanol (PCM), surface modified biotin polyethylene glycol mercapto (Biotin-PEG-SH) was prepared by organic solvent volatilization method to detect the particle size and the amount of drug loaded with polydispersity (PDI),. In vitro cumulative release of (Q). At 37 鈩，

本文编号：2413889