青蒿琥酯对高糖诱导的肾小管上皮细胞TLR4、NF-κB表达及合成的影响

发布时间：2019-02-11 18:48

【摘要】：目的探讨青蒿琥酯(Art)对高糖诱导下的大鼠肾小管上皮细胞(NRK-52E)Toll样受体4(TLR4)、核转录因子-κB(NF-κB)表达及合成的影响。方法体外培养NRK-52E细胞,分为6组:正常对照组、高糖组、Art小剂量组、Art中剂量组、Art高剂量组、依那普利组。培养48 h后收集各组细胞,采用逆转录-聚合酶链反应(RT-PCR)法检测细胞TLR4、NF-κB mRNA含量;采用Western blot法检测细胞TLR4、NF-κB蛋白表达水平。结果 1与正常对照组比较,高糖组细胞TLR4、NF-κB mRNA和蛋白表达水平明显升高(P0.05);2与高糖组比较,Art小、中、高剂量组细胞TLR4、NF-κB mRNA和蛋白表达水平明显降低,且呈剂量依赖性(P0.05);3与依那普利组比较,Art小、中、高剂量组细胞TLR4水平表达均升高,且Art高剂量组与依那普利组差异无统计学意义。与依那普利组比较,Art小、中剂量组细胞NF-κB水平表达升高,Art高剂量组细胞NF-κB水平表达降低,且Art中、高剂量组与依那普利组差异无统计学意义。结论 Art可呈剂量依赖性地抑制高糖环境下NRK-52E细胞TLR4、NF-κB的高表达及合成。
[Abstract]:Objective to investigate the effects of artesunate (Art) on the expression and synthesis of Toll like receptor 4 (TLR4) and nuclear transcription factor 魏 B (NF- 魏 B) in rat renal tubular epithelial cells (NRK-52E) induced by high glucose. Methods NRK-52E cells were cultured in vitro and divided into 6 groups: normal control group, high glucose group, Art small dose group, Art medium dose group, Art high dose group, enalapril group. After 48 h culture, the TLR4,NF- 魏 B mRNA and TLR4,NF- 魏 B protein expression were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot assay respectively. Results 1 the expression of TLR4,NF- 魏 B mRNA and protein in high glucose group was significantly higher than that in normal control group (P0.05). 2Compared with the high glucose group, the expression of TLR4,NF- 魏 B mRNA and protein in the Art group was significantly decreased in a dose-dependent manner (P0.05). 3Compared with enalapril group, the expression of TLR4 in Art group was higher than that in enalapril group, but there was no significant difference between high dose Art group and enalapril group. Compared with enalapril group, the expression of NF- 魏 B in Art group was lower than that in enalapril group, but the expression of NF- 魏 B in high dose Art group was lower than that in Enalapril group. There was no significant difference in Art between high dose group and enalapril group. Conclusion Art can inhibit the expression and synthesis of TLR4,NF- 魏 B in NRK-52E cells in a dose-dependent manner.
【作者单位】：桂林医学院附属医院内分泌科;
【基金】：广西医疗卫生适宜技术研究与开发项目(编号:S201316-07) 广西自然科学基金项目(编号:2012GXNSFAA053085) 广西高等学校计划科研项目(编号:201204LX245)
【分类号】：R96

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