右布洛芬联合盐酸氨基葡萄糖对家兔骨关节的影响
发布时间：2019-02-11 19:02
【摘要】：目的：研究右布洛芬联合盐酸氨基葡萄糖对家兔不同时期骨性关节炎的影响,探讨联合用药的作用机制,为临床研究提供基础。 方法：将84只新西兰兔随机分为7组(n=84)：假手术组(A组)、模型组(B组)、右布洛芬组(C组)、盐酸氨基葡萄糖组(D组)、复方高组(E组)、复方中组(F组)、复方低组(G组),B, C, D, E, F, G组动物均在两侧膝关节进行造模。麻醉方式是耳缘静脉注射20%乌拉坦(5m1/kg,无菌条件下在膝关节侧髌骨旁切口,分离出内侧副韧带,将内侧副韧带切断后再切除掉0.5cm左右长度；内侧半月板全部切除,伤口缝合。A组的造模条件与其它组相同,只是切开关节腔后,不作任何处理,缝合关节腔和皮肤。造模后,每只动物肌肉注射40万单位青霉素防止感染,青霉素连续注射给药3天。C组灌胃给予25mg/kg右布洛芬溶液,D组灌胃给予60mg/kg盐酸氨基葡萄糖,E组灌胃给予25+60mg/kg复方溶液,F组灌胃给予12.5+30mg/kg复方溶液,G组灌胃给予6.25+15mg/kg复方溶液,A、B组灌胃给予0.5%CMC溶液。连续给药120天,于30、120天分批处死动物(30天时每组处死4只,120天时每组处死8只),进行兔膝关节Gotdbrg VM评分；检测血清中IL-1β、TNF-α、ALP水平含量；关节滑液中硫酸软骨素、磷脂酶A2活性的测定；关节软骨HE染色。 结果：兔膝关节Gotdbrg VM评分结果显示,给药30天与120天,A、B组各指标相比均有显著性差异(p0.05),各给药组与B组各指标比较,有显著性差异(p0.05)。给药30天时,血清中IL-1β、TNF-α水平B组明显高于A组、ALP水平B组明显低于A组,有显著性差异(p0.05),给药组经给药后,血清中IL-1β、TNFα含量水平明显低于B组、ALP含量水平明显高于B组,有显著性差异(p0.05)；关节滑液中硫酸软骨素水平B组明显低于A组、磷脂酶A2B组明显高于A组,有显著性差异(p0.05),给药组经给药后,关节滑液中硫酸软骨素水平明显高于B组、磷脂酶A2水平明显低于B组,有显著性差异(p0.05)。关节软骨HE染色结果显示,与模型组比较,C、D、G组关节软骨损伤评分无显著性差异,E、F组具有显著性差异(p0.05)。 结论：右布洛芬联合盐酸氨基葡萄糖能抑制OA中的IL-1β、TNF-α水平,降低磷脂酶A2活性表达,增加OA病情中ALP、硫酸软骨素水平,从而改善OA的症状,促进软骨细胞再生,降低软骨的破坏,进而延缓骨性关节炎的发展。
[Abstract]:Aim: to study the effect of dexibuprofen combined with glucosamine hydrochloride on osteoarthritis in rabbits at different stages, and to explore the mechanism of combined use of dexibuprofen and glucosamine hydrochloride in order to provide a basis for clinical study. Methods: 84 New Zealand rabbits were randomly divided into 7 groups: sham operation group (group A), model group (group B), right ibuprofen group (group C), glucosamine hydrochloride group (group D) and compound high dose group (group E). The middle group (F group) and the low group (G group), B, C, D, E, F, G group) were all made models in bilateral knee joints. The anaesthesia method was to inject 20% uratan (5 ml / kg) into the auricular margin and cut off the medial collateral ligament (medial collateral ligament) under aseptic condition. The medial collateral ligament was cut off after the medial collateral ligament was cut off and the left and right length of the 0.5cm was removed. All medial meniscus were excised and wound sutured. Group A had the same model conditions as other groups, only after incision of articular cavity, without any treatment, suture of articular cavity and skin. After modeling, 400000 units of penicillin were injected intramuscularly to prevent infection in each animal. Penicillin was injected continuously for 3 days. Group C was given 25mg/kg dexbuprofen solution, group D was given 60mg/kg glucosamine hydrochloride. Group E was treated with 25 60mg/kg compound solution, group F with 12.5 30mg/kg compound solution, group G with 6.25 15mg/kg compound solution, group A with 0.5%CMC solution. The rabbits were killed in 30120 days (4 in each group at 30 days and 8 in each group at 120 days), and the Gotdbrg VM score of knee joint was evaluated, and the levels of IL-1 尾, TNF- 伪 and ALP in serum were detected. The activity of chondroitin sulfate and phospholipase A 2 in synovial fluid and HE staining in articular cartilage were determined. Results: the Gotdbrg VM score of knee joint of rabbits showed that there were significant differences between 30 days and 120 days of administration, and there were significant differences between each group and B group (p0.05). After 30 days of administration, the serum levels of IL-1 尾 and TNF- 伪 in group B were significantly higher than those in group A, and the level of ALP in group B was significantly lower than that in group A (p0.05). After administration, the serum levels of IL-1 尾 in group B were significantly higher than those in group A (p0.05). The level of TNF 伪 was significantly lower than that of group B, and the level of ALP was significantly higher than that of group B (p0.05). The level of chondroitin sulfate in synovial fluid in group B was significantly lower than that in group A, and the level of chondroitin sulfate in synovial fluid of group B was significantly higher than that in group B (p0.05). The level of phospholipase A 2 was significantly lower than that of group B (p 0.05). The results of HE staining of articular cartilage showed that there was no significant difference in articular cartilage injury score between the two groups, but there was a significant difference between the two groups (p0.05). Conclusion: dexibuprofen combined with glucosamine hydrochloride can inhibit the levels of IL-1 尾 and TNF- 伪 in OA, decrease the activity of phospholipase A2, increase the level of chondroitin sulfate in ALP, in OA, improve the symptoms of OA and promote the regeneration of chondrocytes. Reduce the destruction of cartilage, and then delay the development of osteoarthritis.
【学位授予单位】：哈尔滨商业大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R684.3
本文编号：2420016
[Abstract]:Aim: to study the effect of dexibuprofen combined with glucosamine hydrochloride on osteoarthritis in rabbits at different stages, and to explore the mechanism of combined use of dexibuprofen and glucosamine hydrochloride in order to provide a basis for clinical study. Methods: 84 New Zealand rabbits were randomly divided into 7 groups: sham operation group (group A), model group (group B), right ibuprofen group (group C), glucosamine hydrochloride group (group D) and compound high dose group (group E). The middle group (F group) and the low group (G group), B, C, D, E, F, G group) were all made models in bilateral knee joints. The anaesthesia method was to inject 20% uratan (5 ml / kg) into the auricular margin and cut off the medial collateral ligament (medial collateral ligament) under aseptic condition. The medial collateral ligament was cut off after the medial collateral ligament was cut off and the left and right length of the 0.5cm was removed. All medial meniscus were excised and wound sutured. Group A had the same model conditions as other groups, only after incision of articular cavity, without any treatment, suture of articular cavity and skin. After modeling, 400000 units of penicillin were injected intramuscularly to prevent infection in each animal. Penicillin was injected continuously for 3 days. Group C was given 25mg/kg dexbuprofen solution, group D was given 60mg/kg glucosamine hydrochloride. Group E was treated with 25 60mg/kg compound solution, group F with 12.5 30mg/kg compound solution, group G with 6.25 15mg/kg compound solution, group A with 0.5%CMC solution. The rabbits were killed in 30120 days (4 in each group at 30 days and 8 in each group at 120 days), and the Gotdbrg VM score of knee joint was evaluated, and the levels of IL-1 尾, TNF- 伪 and ALP in serum were detected. The activity of chondroitin sulfate and phospholipase A 2 in synovial fluid and HE staining in articular cartilage were determined. Results: the Gotdbrg VM score of knee joint of rabbits showed that there were significant differences between 30 days and 120 days of administration, and there were significant differences between each group and B group (p0.05). After 30 days of administration, the serum levels of IL-1 尾 and TNF- 伪 in group B were significantly higher than those in group A, and the level of ALP in group B was significantly lower than that in group A (p0.05). After administration, the serum levels of IL-1 尾 in group B were significantly higher than those in group A (p0.05). The level of TNF 伪 was significantly lower than that of group B, and the level of ALP was significantly higher than that of group B (p0.05). The level of chondroitin sulfate in synovial fluid in group B was significantly lower than that in group A, and the level of chondroitin sulfate in synovial fluid of group B was significantly higher than that in group B (p0.05). The level of phospholipase A 2 was significantly lower than that of group B (p 0.05). The results of HE staining of articular cartilage showed that there was no significant difference in articular cartilage injury score between the two groups, but there was a significant difference between the two groups (p0.05). Conclusion: dexibuprofen combined with glucosamine hydrochloride can inhibit the levels of IL-1 尾 and TNF- 伪 in OA, decrease the activity of phospholipase A2, increase the level of chondroitin sulfate in ALP, in OA, improve the symptoms of OA and promote the regeneration of chondrocytes. Reduce the destruction of cartilage, and then delay the development of osteoarthritis.
【学位授予单位】：哈尔滨商业大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R684.3
【参考文献】
相关期刊论文 前10条
1 郭涛,沈卫星,梁清宇;玻璃酸钠和地塞米松治疗膝骨关节炎临床观察[J];安徽医学;2001年05期
2 吕存贤,王维佳;膝骨关节炎病因病理研究及中医治疗概况[J];浙江中医学院学报;2005年01期
3 伦志坚;叶继英;;腹针结合局部针刺治疗膝关节骨性关节炎[J];广东医学;2006年01期
4 周燕文;徐爱兰;兰聪贤;;盐酸氨基葡萄糖颗粒在健康人体的药代动力学和相对生物利用度[J];中国临床药理学杂志;2008年06期
5 ;人体内氨基葡萄糖的药动学[J];国外医学.药学分册;1994年02期
6 王秀华,苏姿兵,田万斌;丹参注射液对兔骨关节炎软骨的影响[J];辽宁中医杂志;2003年10期
7 冷艳霞;孙璐;邸欣;孙毓庆;;盐酸氨基葡萄糖片的相对生物利用度及其生物等效性评价[J];沈阳药科大学学报;2006年01期
8 卢锋,郭红卫;盐酸氨基葡萄糖对骨关节炎作用机制的初步探讨[J];卫生研究;2003年06期
9 王婧;张忠辉;孙娇梦;许传营;俞雁;韩伟;;大鼠骨关节炎三种动物模型的建立及比较[J];中国细胞生物学学报;2010年03期
10 陈述祥,司徒坚,杨运东,赵崇达,丁林坚,刘红光;自制复方中药对膝骨性关节炎家兔血液流变及病理形态的影响[J];中国临床康复;2005年30期
，本文编号：2420016
本文链接：https://www.wllwen.com/yixuelunwen/yiyaoxuelunwen/2420016.html
最近更新
教材专著
