1-溴丙烷对大鼠大脑线粒体损伤及依达拉奉保护作用的观察

发布时间：2019-02-16 15:29

【摘要】：目的观察1-溴丙烷(1-BP)对大脑线粒体损伤和依达拉奉(EDA)的保护作用。方法将成年雄性Wistar大鼠随机分为对照组、1-BP(800 mg/kg·bw)组、1-BP+1 mg/kg·bw EDA组、1-BP+3 mg/kg·bw EDA组、1-BP+5 mg/kg·bw EDA组、5 mg/kg·bw EDA对照组,每组12只。分别经灌胃和腹腔注射给予1-BP和EDA,1次/d,连续12 d。动物处死后,迅速剥离大脑,采用差速离心法提取大脑前额叶皮层线粒体,检测细胞线粒体复合体Ⅰ、Ⅱ、Ⅲ和Ⅳ活性;测定还原型谷胱甘肽(GSH)和氧化型谷胱甘肽(GSSG)含量,并计算GSH/GSSG比值;采用抗Neu N抗体进行免疫组化染色,计数神经元。结果 1-BP明显降低大脑前额叶皮层细胞线粒体复合体Ⅰ、Ⅱ、Ⅲ、Ⅳ活性(P0.05,P0.01);1-BP组动物大脑皮层GSH含量也较对照组降低(P0.01),GSSG含量升高(P0.01),GSH/GSSG比值明显降低(P0.01);给予1-BP染毒后,大脑前额叶皮层神经元明显减少(P0.01)。1-BP染毒同时给予不同剂量的EDA,大鼠大脑前额叶皮层线粒体复合体活性均有不同程度的升高;GSH含量降低也被明显逆转,GSH/GSSG比值相应升高(P0.05,P0.01);神经元丢失明显改善(P0.05,P0.01);EDA对1-BP诱导大脑损伤的保护作用呈现剂量-效应关系(P0.05)。结论 1-BP导致大脑皮层线粒体损伤,脑内氧化应激及神经元丢失,EDA可有效减轻1-BP神经毒性。
[Abstract]:Objective to observe the protective effect of 1-bromopropane (1-BP) on cerebral mitochondria injury and Edaravone (EDA). Methods Adult male Wistar rats were randomly divided into control group, 1-BP (800 mg/kg bw) group, 1-BP 1 mg/kg bw EDA group, 1-BP 3 mg/kg bw EDA group and 1-BP 5 mg/kg bw EDA group. 5 mg/kg bw EDA control group, 12 rats in each group. 1-BP and EDA,1 were given intraperitoneally and intraperitoneally respectively for 12 days. After the animals were killed, the brain was rapidly stripped and the mitochondria of the prefrontal cortex were extracted by differential centrifugation. The activities of mitochondrial complexes 鈪，

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