高效液相色谱-质谱联用测定小鼠血浆中FG-4592浓度及其药动学研究
发布时间：2019-02-21 16:44
【摘要】：目的建立LC-MS/MS法检测小鼠血浆内FG-4592的浓度,研究FG-4592在小鼠体内的药代动力学。方法KM小鼠腹腔注射FG-4592 5 mg/kg,不同时间点采集血浆,样品经甲醇沉淀。选用Agilent Zorbax SB-Aq-C18色谱柱(2.1 mm×100 mm,3.5μm),柱温25℃,流动相为甲醇-水(含5 mmol/L甲酸铵,0.1%甲酸)(90∶10),流速0.35 m L/min。采用ESI离子源,正离子模式多反应监测,离子通道分别为m/z 353.1→m/z 278.1(FG-4592)和m/z294.9→m/z 235.0(内标物)。结果测定血浆中FG-4592的线性范围为1~1000 ng/m L,日内及日间精密度均10%,准确度在90%~105%范围内,方法学考察均符合生物样品的分析要求。药代动力学参数:Cmax为(22.80±0.36)μg/m L,AUC(0-72)为(31.64±0.97)(μg·h)/m L,t1/2为8.81 h。结论本研究建立的FG-4592血药浓度测定方法可行,该药物在血液中吸收良好,测定结果可为药物的基础研究提供依据。
[Abstract]:Objective to establish a LC-MS/MS method for the determination of FG-4592 in mouse plasma and to study the pharmacokinetics of FG-4592 in mice. Methods KM mice were injected intraperitoneally with FG-4592 5 mg/kg, to collect plasma at different time points, and the samples were precipitated by methanol. The Agilent Zorbax SB-Aq-C18 column (2.1 mm 脳 100 mm,3.5 渭 m),) was used at 25 鈩，
本文编号：2427683
[Abstract]:Objective to establish a LC-MS/MS method for the determination of FG-4592 in mouse plasma and to study the pharmacokinetics of FG-4592 in mice. Methods KM mice were injected intraperitoneally with FG-4592 5 mg/kg, to collect plasma at different time points, and the samples were precipitated by methanol. The Agilent Zorbax SB-Aq-C18 column (2.1 mm 脳 100 mm,3.5 渭 m),) was used at 25 鈩，
本文编号：2427683
本文链接：https://www.wllwen.com/yixuelunwen/yiyaoxuelunwen/2427683.html
最近更新
教材专著
