抗结核海洋放线菌的筛选及菌株HY286生物活性研究

发布时间：2019-02-22 15:02

【摘要】：海洋放线菌是研究抗结核药物及其先导化合物的重要来源。以耻垢分枝杆菌(Mycobacterium smegmatis,Msm)作为指示菌,采用平板对峙法,从漳州浅滩泥土分离的77株放线菌中筛选出9株对Msm具有抑制作用的抗性放线菌,其中1株对Msm具有高效抑制作用的拮抗放线菌HY286。通过对菌株HY286的菌落形态观察、生理生化鉴定及16S r DNA序列分析,初步鉴定其为一株马杜拉属放线菌。通过测定菌株HY286发酵产物的抗耻垢分枝杆菌MIC值、抗菌活性和细胞毒活性。结果表明,该菌株产生化合物的抗耻垢分枝杆菌MIC值为200μg/m L,具有较好的抗结核分枝杆菌的潜力,且对枯草芽孢杆菌、短小芽孢杆菌也具有较好的抑制作用,对He La和Hep G2的抑制率分别为89.3%和94.2%。
[Abstract]:Marine actinomycetes are important sources of antituberculous drugs and their lead compounds. Using Mycobacterium smegmatis,Msm as indicator, 9 resistant actinomycetes were screened from 77 strains of actinomycetes isolated from soil of Zhangzhou shoal by plate confrontation method. One of them is an antagonistic actinomycetes HY286., which has high inhibitory effect on Msm. The colony morphology, physiological and biochemical identification and 16s r DNA sequence analysis of the strain HY286 were preliminarily identified as an actinomycetes of Madura. The MIC value, antimicrobial activity and cytotoxic activity of the fermentation product of HY286 were determined. The results showed that the MIC value of the compound produced by the strain was 200mg / L, which had a good potential for anti-Mycobacterium tuberculosis, and also had a good inhibitory effect on Bacillus subtilis and Bacillus brevis, and it had a good inhibitory effect on Bacillus subtilis and B. brevis, and had a good inhibitory effect on Bacillus subtilis and B. brevis. The inhibition rates of He La and Hep G2 were 89. 3% and 94. 2%, respectively.
【作者单位】：陕西省微生物研究所微生物资源中心;
【基金】：陕西省科技厅项目(2016FWPT-10) 陕西省科学院科技计划项目(2014k-35-2)
【分类号】：R915

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