载RGD肽脂质体的优化和初步评价
发布时间：2019-02-25 20:27
【摘要】：采用逆相蒸发法制备了载RGD肽脂质体。以包封率为指标,采用单因素试验和正交设计优化工艺条件。确定的优化条件为:卵磷脂和胆固醇的质量比为4∶1,磷酸盐缓冲液的体积为10 ml,RGD肽的加入量为18 mg,超声时间为3 min。3批优化制品平均粒径为473 nm,平均包封率为75.35%。RGD肽脂质体在磷酸盐缓冲液中16 h累积释放率为48.5%,相较于RGD肽溶液有一定的缓释效果。初步稳定性试验表明,载RGD肽脂质体在4℃保存相对较稳定。
[Abstract]:RGD peptide liposomes were prepared by reverse phase evaporation. The single factor test and orthogonal design were used to optimize the technological conditions with the inclusion efficiency as the index. The optimum conditions are as follows: the mass ratio of lecithin to cholesterol is 4 渭 1, the volume of phosphate buffer is 10 ml,RGD peptide, the dosage of peptide is 18 mg, the ultrasonic time is 3 min.3, the average particle size of the batch optimized product is 473 nm,. The average entrapment efficiency of 75.35%.RGD peptide liposomes was 48.5% in phosphate buffer solution for 16 h, which was more effective than that of RGD peptide solution. The preliminary stability test showed that the RGD-loaded peptide liposomes were relatively stable at 4 鈩，
本文编号：2430508
[Abstract]:RGD peptide liposomes were prepared by reverse phase evaporation. The single factor test and orthogonal design were used to optimize the technological conditions with the inclusion efficiency as the index. The optimum conditions are as follows: the mass ratio of lecithin to cholesterol is 4 渭 1, the volume of phosphate buffer is 10 ml,RGD peptide, the dosage of peptide is 18 mg, the ultrasonic time is 3 min.3, the average particle size of the batch optimized product is 473 nm,. The average entrapment efficiency of 75.35%.RGD peptide liposomes was 48.5% in phosphate buffer solution for 16 h, which was more effective than that of RGD peptide solution. The preliminary stability test showed that the RGD-loaded peptide liposomes were relatively stable at 4 鈩，
本文编号：2430508
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