液相色谱-串联质谱法测定连续肾脏替代治疗患者血浆及超滤液中头孢哌酮与舒巴坦浓度

发布时间：2019-03-04 10:10

【摘要】：目的建立液相色谱-串联质谱法(LC-MS/MS)测定连续肾脏替代治疗(continuous renal replacement therapy,CRRT)患者血浆及超滤液中头孢哌酮与舒巴坦浓度。方法以头孢呋辛酯为内标,采用Waters Atlantis d C18色谱柱(150 mm×4.6mm,5.0μm)进行分离,通过串联质谱仪,以多反应监测方式进行测定。用于定量分析的离子对分别是m/z 644.1→528.1(头孢哌酮)、m/z 231.8→188.0(舒巴坦)和m/z 509.3→206.9〔头孢呋辛酯,内标(IS)〕。结果头孢哌酮在10~500μg/ml范围内,舒巴坦在6~300μg/ml范围内线性关系良好。提取回收率90.0%,日内和日间相对标准偏差均15%。血浆和超滤液的基质效应结果表明,两种介质的基质对头孢哌酮、舒巴坦和IS的测定影响较小。结论本方法简便、快捷、灵敏度高,可在同一样品中同时检测两种药物,适用于危重患者行CRRT时血药浓度、超滤液药物浓度的监测,计算筛选系数(sieving coefficient,Sc),为剂量调整提供准确依据。
[Abstract]:Objective To establish liquid chromatography-tandem mass spectrometry (LC-MS/ MS) to measure the concentration of ceftriaxone and sulbactam in plasma and ultrafiltrate of continuous renal replacement therapy (CRRT). The method was carried out by using a Waters Atlantis d C18 column (150 mm, 4.6 mm, 5.0. mu.m) as an internal standard and measured by a tandem mass spectrometer in a multi-reactive monitoring manner. The ion pairs for the quantitative analysis were m/ z 644.1-528.1 (ceftrione), m/ z 231.8-188.0 (sulbactam) and m/ z 509.3-206.9% of dioctyl-cephem, internal standard (IS), respectively. Results The linear relationship of sulbactam in the range of 10-500 & mu; g/ ml and sulbactam in the range of 6-300 & mu; g/ ml was good. The extraction recovery was 90.0%, and the relative standard deviation of day and day was 15%. The matrix effect of plasma and ultrafiltrate shows that the matrix of the two media has less influence on the determination of ceftrione, sulbactam and IS. Conclusion The method is simple, rapid and high in sensitivity, and can be used for simultaneous detection of two drugs in the same sample. The method is applicable to the monitoring of the concentration of the drug in the critical patients with CRRT, the monitoring of the drug concentration of the ultrafiltrate, and the calculating and screening coefficient (Sc), which provides an accurate basis for dose adjustment.
【作者单位】：安徽医科大学解放军306临床学院;军事医学科学院附属医院临床药理室;中国人民解放军第306医院;
【分类号】：R96

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