肾上腺髓质素对大鼠肢体缺血再灌注后心肌损伤和细胞凋亡的影响

发布时间：2019-03-25 18:38

【摘要】：目的观察大鼠肢体缺血再灌注后心肌细胞凋亡情况,探讨外源性肾上腺髓质素(ADM)对大鼠肢体缺血再灌注(LIR)后心肌损伤的保护机制。方法健康雄性Wistar大鼠30只,随机分成对照组(Control组)、肢体缺血再灌注4 h组(LIR组)和ADM+LIR组(ADM组),每组10只。生化法检测血浆心肌酶含量;比色法测定心肌组织丙二醛(MDA)和超氧化物歧化酶(SOD)含量的变化;免疫组织化学法检测心肌组织凋亡蛋白酶Caspase-3蛋白表达情况;原位末端脱氧核糖核苷酸转移酶标记(TUNEL)技术检测心肌凋亡细胞。结果与LIR组比较,心肌酶含量下降,心肌组织MDA含量减少,SOD活性增强(P0.01);心肌组织Caspse-3蛋白表达下降,心肌细胞凋亡指数明显下降(P0.01)。结论 ADM可减轻大鼠LIR后心肌损伤,其机制可能与抑制心肌氧化应激与细胞凋亡有关。
[Abstract]:Aim to observe the apoptosis of cardiomyocytes after limb ischemia-reperfusion in rats and to explore the protective mechanism of exogenous adrenomedullin (ADM) on myocardial injury after ischemia-reperfusion (LIR) in limbs of rats. Methods Thirty healthy male Wistar rats were randomly divided into three groups: control group (Control group), limb ischemia-reperfusion group (LIR group) and ADM LIR group (ADM group), with 10 rats in each group. Plasma myocardial enzyme content was measured by biochemical method, malondialdehyde (MDA) and superoxide dismutase (SOD) content were measured by colorimetric method, expression of apoptotic protease Caspase-3 protein was detected by immunohistochemical method. Cardiomyocyte apoptosis was detected by in situ terminal deoxynucleotidyl transferase labeled (TUNEL) technique. Results compared with LIR group, the content of myocardial enzyme decreased, the content of MDA decreased and the activity of SOD increased (P0.01), the expression of Caspse-3 protein decreased and the index of cardiomyocyte apoptosis decreased significantly (P0.01). Conclusion ADM can reduce myocardial injury after LIR, and its mechanism may be related to the inhibition of myocardial oxidative stress and apoptosis.
【作者单位】：唐山职业技术学院基础医学部生理学教研室;唐山市开滦总医院ICU;
【基金】：唐山市科学技术研究与发展项目(No.12130239b)
【分类号】：R965

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