αB-晶状体蛋白对急性高眼压大鼠视网膜神经节细胞保护作用机制的研究

发布时间：2019-03-30 20:19

【摘要】：目的研究αB-晶状体蛋白对钾离子通道kv1.1、kv1.3及凋亡抑制因子Bcl-xl和凋亡因子Caspase-3的影响,探讨αB-晶状体蛋白对急性高眼压大鼠视网膜神经节细胞(retinal ganglion cells,RGC)保护作用的机制。方法 60只SD大鼠随机分为A组(急性高眼压组)、B组(急性高眼压+玻璃体内注射生理盐水5μL组)、C组(急性高眼压+玻璃体内注射10×10-6 g·L-1αB-晶状体蛋白5μL组),每组20只,右眼为实验眼。注射后7 d和14 d时,应用Western blot和实时定量逆转录聚合酶链反应(QRT-PCR)法检测kv1.1、kv1.3、Bcl-xl和Caspase-3在视网膜上的表达水平。结果注射后7 d和14 d时,C组kv1.1、kv1.3通道蛋白和凋亡因子Caspase-3蛋白表达水平和mRNA相对表达量均较A组、B组低,差异均有统计学意义(均为P0.05);C组Bcl-xl蛋白表达水平和mRNA相对表达量较A组、B组高,差异均有统计学意义(均为P0.05);相同时间点不同因子A组、B组间两两比较,差异均无统计学意义(均为P0.05)。结论αB-晶状体蛋白通过抑制急性高眼压后RGC上钾离子通道kv1.1、kv1.3的表达,从而提高凋亡抑制因子Bcl-xl的表达、降低凋亡因子Caspase-3的表达,减少细胞凋亡,保护RGC。
[Abstract]:Objective to study the effects of 伪 B-crystallin on potassium channel kv1.1,kv1.3, apoptosis inhibitor Bcl-xl and apoptosis factor Caspase-3, and to explore the effect of 伪 B-crystallin on (retinal ganglion cells, in retinal ganglion cells of rats with acute ocular hypertension. RGC) the mechanism of protective effect. Methods Sixty SD rats were randomly divided into group A (), B group with acute hyperocular pressure, group 5 渭 L with intravitreal injection of normal saline). Group C (10 脳 10 g 路L-1 伪 B-crystallin 5 渭 L) received intravitreal injection of 10 脳 10 脳 10 g 路L-1 伪 B-crystallin in group C with 20 eyes in each group, and the right eye was the experimental eye. Western blot and real-time quantitative reverse transcription polymerase chain reaction (QRT-PCR) were used to detect the expression of kv1.1,kv1.3,Bcl-xl and Caspase-3 in retina 7 d and 14 d after injection. Results on the 7th and 14th day after injection, the expression level of kv1.1,kv1.3 channel protein and apoptosis factor Caspase-3 protein and the relative expression of mRNA in group C were lower than those in group A and group B (P 0.05). The expression level of Bcl-xl protein and the relative expression of mRNA in group C were significantly higher than those in group A and group B (P 0.05). At the same time point, there was no significant difference between group A and group B in different factors (P 0.05). Conclusion 伪 B-crystallin can increase the expression of inhibitor of apoptosis factor Bcl-xl and decrease the expression of apoptosis factor Caspase-3 and decrease apoptosis by inhibiting the expression of potassium channel kv1.1,kv1.3 in RGC after acute ocular hypertension. Protect RGC.
【作者单位】：武警总医院眼科;
【基金】：中国博士后科学基金编号:20080430444)~~
【分类号】：R965

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