关附甲素抗氧化和抗房颤作用

发布时间：2019-04-02 03:40

【摘要】：研究关附甲素(Guanfu base A,GFA)的抗房颤和抗氧化作用。大鼠尾静脉注射乙酰胆碱-氯化钙混合液(Ca Cl210 mg/m L,Ach 66μg/m L),连续7 d建立房颤模型。SD大鼠分为正常组、模型组、GFA治疗组(6 mg/kg,12 mg/kg)、胺碘酮(Amiodarone,Ami)治疗组(50 mg/kg)以及洛伐他汀(Lovastatin,Lov)治疗组(10 mg/kg)。测定大鼠房颤持续时间和有效不应期(AERP),用实时荧光定量PCR方法和Western blot方法测定氧化应激相关基因表达,试剂盒测定抗氧化酶的活性。结果显示:与模型组相比,GFA(6 mg/kg,12 mg/kg,po)治疗4 d后能缩短房颤持续时间,延长AERP,超氧化物歧化酶的活力提高,丙二醛含量明显下降。心房肌p22phox、p47phox、p67phox、gp91phox表达下调,膜Rac-1与胞浆Rac-1比值显著下降,缝隙连接蛋白(connexin40)表达升高。提示GFA(6 mg/kg,12 mg/kg)能有效抑制房颤引起的大鼠心房氧化应激损害,抑制房颤发生。
[Abstract]:To study the anti-atrial fibrillation and antioxidation effects of Guanfu A (Guanfu base A). The rat model of atrial fibrillation was established by intravenous injection of acetylcholine-calcium chloride mixed solution (Ca Cl210 mg/m L, Ach 66ug / m L),) for 7 days. SD rats were divided into normal group, model group and GFA treatment group (6 mg/kg,12 mg/kg). Amiodarone (Amiodarone,Ami) group (50 mg/kg) and lovastatin (Lovastatin,Lov) group (10 mg/kg). The duration of atrial fibrillation (AF) and the effective refractory period (AERP),) of rats were measured by real-time fluorescence quantitative PCR and Western blot, and the activity of antioxidant enzymes was measured by Elisa. The results showed that compared with the model group, GFA (6 mg/kg,12 mg/kg,po could shorten the duration of atrial fibrillation, prolong the activity of AERP, superoxide dismutase (SOD) and decrease the content of malondialdehyde (MDA). The expression of p22phoxs, p47phoxs, p67phoxs and gp91phox was down-regulated, the ratio of membrane Rac-1 to cytoplasmic Rac-1 was significantly decreased, and the expression of gap junction protein (connexin40) was increased. It is suggested that GFA (6 mg/kg,12 mg/kg) can effectively inhibit atrial oxidative stress damage induced by atrial fibrillation and inhibit the occurrence of atrial fibrillation in rats.
【作者单位】：中国药科大学临床药学教研室;
【基金】：国家“重大新药创制”科技重大专项课题资助项目(No.2009ZX09103-345)~~
【分类号】：R965

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