负载多西紫杉醇白及胶的制备及其抗肿瘤作用的初步研究
发布时间：2019-04-08 08:56
【摘要】：目的:采用具有良好的生物相容性、自身降解性、无刺激性、缓释性的天然高分子材料白及胶作为药物载体,制备多西紫杉醇白及胶,并初步研究其腹腔注射给药的抗肿瘤作用。方法:1、取适量中药饮片白及低温干燥,采用水提醇沉法获得白及粗多糖,运用硫酸-蒽醌法测定其中的粗多糖含量,将干燥的白及多糖溶于纯水,并采用物理方法制备负载多西紫杉醇的白及胶。通过扫描电镜观察白及胶中多西紫杉醇的分布情况,并进行体外释放实验。2、建立荷小鼠肝癌H22腹水模型,造模24小时后随机分为3组,即生理盐水对照组、多西紫杉醇注射液组、多西紫杉醇白及胶组,每组5只;其中多西紫杉醇注射液组和多西紫杉醇白及胶组分别予多西紫杉醇10mg/kg剂量腹腔注射给药,生理盐水对照组给予等体积生理盐水腹腔注射给药,共给药4次,给药间隔72小时,期间观察小鼠的一般情况,于用药后第15天后处死小鼠,收集腹水,计算腹水量,观察腹腔转移的情况,取多西紫杉醇白及胶组小鼠的重要脏器,石蜡包埋切片行HE染色后观察各脏器组织损伤情况。结果:1、所提取的白及粗多糖中粗多糖含量为67.26%,扫描电镜发现多西紫杉醇白及胶中多西紫杉醇分布较均匀,体外释放实验表明多西紫杉醇白及胶呈缓慢释放过程,其在150h后约释放45%多西紫杉醇;2、在给药后第10天,生理盐水对照组测量小鼠体重,较多西紫杉醇注射液组及多西紫杉醇白及胶组明显增加(P0.05);多西紫杉醇白及胶组较生理盐水对照组比较,腹水量明显减少(P0.05);同时肉眼观察各组小鼠肠系膜上的癌结节,多西紫杉醇白及胶组较生理盐水对照组及多西紫杉醇注射液组减少;多西紫杉醇白及胶组小鼠的心肝脾肺肾HE染色切片电子显微镜下观察后未见明显组织损伤。结论:负载多西紫杉醇的白及胶制备简便,药物分布较均匀,并具有一定的缓释性。腹腔注射给药显示出具有一定的体内抗肿瘤效果,值得进一步研究。
[Abstract]:Objective: to prepare polycedar alcohol white and gum with good biocompatibility, self-degradability, non-irritant and slow-release natural macromolecule material white and gum as drug carrier. The antineoplastic effect of intraperitoneal injection was also studied. Methods: 1, the crude polysaccharide was obtained by water extraction and alcohol precipitation method, and the content of crude polysaccharide was determined by sulfuric acid-anthraquinone method, and the dried white and polysaccharide were dissolved in pure water, the dried white and polysaccharide were dissolved in pure water, and the content of crude polysaccharide was determined by sulfuric acid-anthraquinone method. The white and adhesive loaded with docetaxel were prepared by physical method. The distribution of docetaxel in white and jelly was observed by scanning electron microscope (SEM), and the release experiment in vitro was carried out. (2) the ascites model of mice bearing hepatoma H22 was established and divided into three groups at random 24 hours after establishment of the model, namely, saline control group. Docetaxel injection group, docetaxel white and glue group, 5 rats in each group; Among them, docetaxel injection group and docetaxel white group and gel group were injected intraperitoneally with docetaxel 10mg/kg, and normal saline control group were injected intraperitoneally with equal volume saline for 4 times, and the dose of docetaxel injection group and doxorubicin group were 4 times. The mice were killed on the 15th day after administration, the ascites was collected, the ascites volume was calculated, the peritoneal metastasis was observed, and the important organs of the mice in the doxorubicin group were taken from the mice treated with doxorubicin and gum. Paraffin embedded sections were stained with HE to observe the injury of each organ. The results were as follows: 1. The content of crude polysaccharide was 67.26%. SEM showed that the distribution of docetaxel in the white and gum of Taxodium dosicae was more uniform. The in vitro release test showed that the white and gum of Taxodium dosiliensis showed slow release process. About 45% docetaxel was released after 150 h. 2. On the 10th day after administration, the body weight of mice in the saline control group was significantly higher than that in the docetaxel injection group and docetaxel white and colloid groups (P0.05). Compared with the saline control group, the volume of ascites in the doxorubicin group and the colloid group decreased significantly (P0.05). At the same time, the tumor nodules on mesentery of mice in each group were observed by naked eye, the number of doxorubicin white and colloid group was lower than that of normal saline control group and docetaxel injection group. No obvious tissue damage was observed under electron microscope in the HE stained sections of heart, liver, spleen, lung and kidney of mice treated with doxorubicin and doxorubicin. Conclusion: the preparation of docetaxel-loaded white and gum is simple, the drug distribution is uniform, and it has a certain slow-release. Intraperitoneal injection showed a certain anti-tumor effect in vivo, which is worthy of further study.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R943;R96
本文编号：2454455
[Abstract]:Objective: to prepare polycedar alcohol white and gum with good biocompatibility, self-degradability, non-irritant and slow-release natural macromolecule material white and gum as drug carrier. The antineoplastic effect of intraperitoneal injection was also studied. Methods: 1, the crude polysaccharide was obtained by water extraction and alcohol precipitation method, and the content of crude polysaccharide was determined by sulfuric acid-anthraquinone method, and the dried white and polysaccharide were dissolved in pure water, the dried white and polysaccharide were dissolved in pure water, and the content of crude polysaccharide was determined by sulfuric acid-anthraquinone method. The white and adhesive loaded with docetaxel were prepared by physical method. The distribution of docetaxel in white and jelly was observed by scanning electron microscope (SEM), and the release experiment in vitro was carried out. (2) the ascites model of mice bearing hepatoma H22 was established and divided into three groups at random 24 hours after establishment of the model, namely, saline control group. Docetaxel injection group, docetaxel white and glue group, 5 rats in each group; Among them, docetaxel injection group and docetaxel white group and gel group were injected intraperitoneally with docetaxel 10mg/kg, and normal saline control group were injected intraperitoneally with equal volume saline for 4 times, and the dose of docetaxel injection group and doxorubicin group were 4 times. The mice were killed on the 15th day after administration, the ascites was collected, the ascites volume was calculated, the peritoneal metastasis was observed, and the important organs of the mice in the doxorubicin group were taken from the mice treated with doxorubicin and gum. Paraffin embedded sections were stained with HE to observe the injury of each organ. The results were as follows: 1. The content of crude polysaccharide was 67.26%. SEM showed that the distribution of docetaxel in the white and gum of Taxodium dosicae was more uniform. The in vitro release test showed that the white and gum of Taxodium dosiliensis showed slow release process. About 45% docetaxel was released after 150 h. 2. On the 10th day after administration, the body weight of mice in the saline control group was significantly higher than that in the docetaxel injection group and docetaxel white and colloid groups (P0.05). Compared with the saline control group, the volume of ascites in the doxorubicin group and the colloid group decreased significantly (P0.05). At the same time, the tumor nodules on mesentery of mice in each group were observed by naked eye, the number of doxorubicin white and colloid group was lower than that of normal saline control group and docetaxel injection group. No obvious tissue damage was observed under electron microscope in the HE stained sections of heart, liver, spleen, lung and kidney of mice treated with doxorubicin and doxorubicin. Conclusion: the preparation of docetaxel-loaded white and gum is simple, the drug distribution is uniform, and it has a certain slow-release. Intraperitoneal injection showed a certain anti-tumor effect in vivo, which is worthy of further study.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R943;R96
【参考文献】
相关期刊论文 前10条
1 蒋建新;刘彦涛;周自圆;段久芳;孙达峰;;高分子多糖水凝胶功能材料研究与应用进展[J];林产化学与工业;2017年02期
2 徐杉;吴敬波;傅少志;;水凝胶在抗肿瘤药物中的研究进展[J];中国现代应用药学;2016年05期
3 吕洪乐;张同华;李倩;;白及多糖药理作用的研究进展[J];中国药房;2015年28期
4 王凤凤;郑根建;周岚;;生物水凝胶的现状与展望[J];海南医学;2015年17期
5 王慧;宋福来;刘万顺;韩宝芹;;壳聚糖基水凝胶对肿瘤在体内外生长的影响研究[J];中国海洋大学学报(自然科学版);2015年03期
6 台琪瑞;殷秀惠;;白及多糖的提取与分析[J];中国药业;2014年22期
7 朱海涛;楚亚琴;陈黎;;正交试验优选蒽酮-硫酸法测定白芨多糖含量条件[J];中国医院药学杂志;2014年20期
8 刘继威;魏晓东;唐艳萍;;白及多糖及其与5-Fu联用对人胃癌MKN45细胞株的影响[J];内蒙古中医药;2014年05期
9 代民涛;柴可夫;李秀月;;《金匮要略》虚劳病八方探略[J];中医学报;2014年02期
10 罗仕华;郑传胜;黎维勇;卫乐乐;王勇;夏向文;周国锋;冯敢生;;白及多糖体外抗肿瘤实验研究[J];中成药;2014年01期
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