山梨酸钾和D-异抗坏血酸钠联合作用HepG2细胞的生物学效应分析
发布时间：2019-04-17 06:40
【摘要】：该文研究食品添加剂联合作用人肝癌HepG2细胞后的多参数生物学指标,并探索可能存在的损伤机制。CCK-8法检测受试物山梨酸钾及D-异抗坏血酸钠0.13~2.00 g/L分别作用HepG2细胞24、48及72 h后的细胞增殖活力,显示山梨酸钾及D-异抗坏血酸钠均呈显著的时间、剂量依赖性的抑制HepG2细胞增殖,其24 h的IC50分别为1.35±0.11 g/L和1.58±0.17 g/L。高内涵分析结果表明,与单独组相比,联合组显著降低细胞数量和线粒体膜电位,增大细胞膜通透性及活性氧水平(P0.05);高剂量联合组(0.30+0.41 g/L)显著增加DNA损伤(P0.01),表现为协同作用。qRT-PCR和Western印迹法显示,山梨酸钾及D-异抗坏血酸钠可显著提高P53、Caspase-3、Bax、γ-H2AX表达,同时显著降低Bcl-2表达,从而抑制HepG2细胞增殖。
[Abstract]:In this paper, the multi-parameter biological indexes of human hepatoma HepG2 cells treated with food additives were studied. The proliferation of HepG2 cells treated with potassium sorbate and sodium D-isoascorbate 0.13 and 2.00 g / L for 24 h, 48 h and 72 h, respectively, was detected by CCK-8 assay. The results showed that potassium sorbate and sodium D-isoascorbate inhibited the proliferation of HepG2 cells in a dose-dependent manner. The IC50 for 24 h were 1.35 卤0.11 g / L and 1.58 卤0.17 g / L, respectively. The results of high-connotation analysis showed that the combination group significantly decreased the number of cells and mitochondrial membrane potential, increased the permeability of cell membrane and the level of reactive oxygen species (P05). In high dose combination group (0.30 0.41 g / L), DNA damage was significantly increased (P0.01). QRT-PCR and Western blot showed that potassium sorbate and D-ascorbate significantly increased P53, caspase-3, Bax, and so on. The expression of 纬-H2AX and the decrease of Bcl-2 expression could inhibit the proliferation of HepG2 cells.
【作者单位】： 浙江工商大学食品与生物工程学院;
【基金】：卓越食品人才科技培育项目(No.20151207)资助~~
【分类号】：R99;TS202.3
[Abstract]:In this paper, the multi-parameter biological indexes of human hepatoma HepG2 cells treated with food additives were studied. The proliferation of HepG2 cells treated with potassium sorbate and sodium D-isoascorbate 0.13 and 2.00 g / L for 24 h, 48 h and 72 h, respectively, was detected by CCK-8 assay. The results showed that potassium sorbate and sodium D-isoascorbate inhibited the proliferation of HepG2 cells in a dose-dependent manner. The IC50 for 24 h were 1.35 卤0.11 g / L and 1.58 卤0.17 g / L, respectively. The results of high-connotation analysis showed that the combination group significantly decreased the number of cells and mitochondrial membrane potential, increased the permeability of cell membrane and the level of reactive oxygen species (P05). In high dose combination group (0.30 0.41 g / L), DNA damage was significantly increased (P0.01). QRT-PCR and Western blot showed that potassium sorbate and D-ascorbate significantly increased P53, caspase-3, Bax, and so on. The expression of 纬-H2AX and the decrease of Bcl-2 expression could inhibit the proliferation of HepG2 cells.
【作者单位】： 浙江工商大学食品与生物工程学院;
【基金】：卓越食品人才科技培育项目(No.20151207)资助~~
【分类号】：R99;TS202.3
【相似文献】
相关期刊论文 前10条
1 王修华,刘敏,丁兆东;山梨酸的生产及市场[J];精细石油化工进展;2001年03期
2 赵建生;山梨酸钾在火腿肠中的应用研究[J];肉类工业;2003年01期
3 刘达玉,游见明;山梨酸钾保藏甜橙汁的评价研究[J];中国食品添加剂;2003年03期
4 仝其根,周敏,于同泉,陆平;固相法合成山梨酸钾可行性研究[J];食品工业科技;2005年10期
5 刘国信;;山梨酸对禽肉的保鲜研究[J];肉类工业;2006年01期
6 李自强;林l司，
本文编号：2459192
本文链接：https://www.wllwen.com/yixuelunwen/yiyaoxuelunwen/2459192.html
最近更新
教材专著
