纳米氧化铜对豚鼠血脑屏障通透性影响观察

发布时间：2019-04-18 08:07

【摘要】：目的观察40和200 nm 2种粒径纳米氧化铜颗粒(Cu ONPs)对豚鼠血脑屏障通透性造成的影响。方法制备粒径为40和200 nm的近球型Cu ONPs用于测试。以0.5%HPMC作为分散剂配制Cu ONPs染毒分散悬液。选取250~300 g的豚鼠用于试验。试验设40和200 nm Cu ONPs组、Cu SO4组、0.5%HPMC组和生理盐水组,每组5只动物。以一次性舌下静脉注射方式染毒。Cu ONPs组、Cu SO4组、HPMC组和生理盐水组染毒剂量分别按照1.5、65、4和4 ml/kg·bw进行。分别于染毒后24和48 h从每组取5只动物,先经舌下静脉给动物注射2%伊文氏蓝(EB)生理盐水溶液(4 ml/kg),1 h后实施心脏灌注,切取大脑顶叶皮质及皮质下结构组织,以脑组织中EB渗入量(以吸光度A值计)表示动物血脑屏障的开放程度。结果 40 nm Cu ONP作用24和48 h时皮质和皮质下结构组织A值明显高于相应200 nm Cu ONP组和生理盐水组A值差异有统计学意义(P0.05)。2种粒径Cu ONPs组作用48 h的A值较各自作用24 h的A值明显增大差异有统计学意义(P0.05)。同时,200 nm Cu ONPs组48 h皮质A值(0.383±0.017 A/mg)与生理盐水组A值(0.103±0.021 A/mg)之间差异有统计学意义(P0.05)。除Cu SO4组24和48 h皮质下结构A值分别为0.087±0.026和0.071±0.031 A/mg高于对应生理盐水组,分别为0.041±0.013和0.069±0.022 A/mg外,其余各组的A值与生理盐水组间差异均无统计学意义(P0.05)。结论 40 nm Cu ONP具有显著的致豚鼠血脑屏障开放效应,且这种效应在作用24~48 h具有增强特点。
[Abstract]:Objective to observe the effect of 40 and 200 nm nano-sized copper oxide particles (Cu ONPs) on the permeability of blood-brain barrier in guinea pigs. Methods the near spherical Cu ONPs with particle size of 40 nm and 200 nm was prepared for testing. The dispersion suspension of Cu ONPs was prepared by using 0.5%HPMC as dispersant. A guinea pig of 250 g 300 g was selected for the experiment. Five animals in each group were divided into four groups: 40 and 200 nm Cu ONPs groups, Cu SO4 group, 0.5%HPMC group and saline group. . Cu ONPs group, Cu SO4 group, HPMC group and saline group were administrated according to 1.5 ml/ kg 路bw, 65 ml/ kg 路bw, 4 ml/ kg 路bw and 4 ml/ kg 路bw, respectively. Five animals in each group were taken from each group at 24 and 48 h after exposure. The animals were injected with 2% Evans blue (EB) saline solution (4 ml/kg) through the sublingual vein, and then cardiac perfusion was performed 1 hour later. The parietal lobe cortex and subcortical structure tissue were removed and the opening degree of blood-brain barrier (BBB) was indicated by the amount of EB infiltration in brain tissue (measured by absorbance A value). Results the A value of cortex and subcortical structure tissue exposed to 40 nm Cu ONP for 24 and 48 h was significantly higher than that of the corresponding 200 nm Cu ONP group and physiological saline group (P0.05). The A values of the two Cu ONPs groups treated for 48 h were significantly higher than those of the control group (P0.05). There was significant difference in A value between the two groups at 24 h (P0.05). At the same time, the cortical A value of 200 nm Cu ONPs group (0.383 卤0.017 A/mg) was significantly different from that of physiological saline group (0.103 卤0.021 A/mg) (P0.05). Except the A value of subcortical structure in Cu SO4 group was 0.087 卤0.026 and 0.071 卤0.031 A/mg higher than that in normal saline group (0.041 卤0.013 and 0.069 卤0.022 A/mg) at 24 and 48 hours, respectively. There was no significant difference between the other groups in A value and saline group (P0.05). Conclusion 40 nm Cu ONP has a significant open effect on blood-brain barrier in guinea pigs, and this effect can be enhanced at 24 h and 48 h.
【作者单位】：中国疾病预防控制中心环境与健康相关产品安全所环境毒理室;北京工业大学环境与能源工程学院;
【基金】：国家自然科学基金(81372949)
【分类号】：R99

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