HPLC法同时快速测定伐昔洛韦、泛昔洛韦和更昔洛韦含量

发布时间：2019-04-24 03:54

【摘要】：目的建立同时快速测定伐昔洛韦、泛昔洛韦和更昔洛韦3种化合物含量的分析方法。方法采用C18色谱柱,流动相:甲醇-0.03mol/L磷酸二氢铵溶液(用磷酸调节p H值至3.0±0.1)(V:V=35:65);流速:1.0m L/min,柱温:35℃,检测波长为250nm,结果更昔洛韦、伐昔洛韦和泛昔洛韦浓度分别在9.88~59.28、9.48~56.88和20.08~120.48μg/m L范围内,与其峰面积呈良好线性关系,线性方程分别为:A=6.71×104C+7.91×104(R2=0.9997),A=4.89×104C+6.94×104(R2=0.9997)和A=1.06×104C+3.82×104(R2=0.9995);加标回收率分别为99.7%(RSD为1.0%)、100.4%(RSD为1.2%)和9.3%(RSD为1.1%)(n=9);样品溶液在6h内稳定。结论建立的方法简便、准确,可同时测定核苷类抗病毒药物的含量。
[Abstract]:Objective to establish a rapid method for simultaneous determination of valaciclovir, fanciclovir and ganciclovir. Methods A C18 column was used with a mobile phase of methanol-0.03mol/L ammonium dihydrogen phosphate solution (adjusted pH value to 3.0 卤0.1 with phosphoric acid) (V:V=35:65). The flow rate was 1.0 mL / min, the column temperature was 35 鈩，

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