人参皂苷CK联合5-氟尿嘧啶对人胰腺癌PANC-1细胞的增殖、凋亡及上皮间质转化的影响

发布时间：2019-04-24 07:33

【摘要】：目的:研究人参皂苷CK联合5-氟尿嘧啶(5-FU)对人胰腺癌PANC-1细胞的增殖、凋亡及上皮间质转化(EMT)的影响。方法:将对数生长期的PANC-1细胞分为空白对照组、人参皂苷CK组(30 mg/L)、5-FU组(25 mg/L)和联用组(人参皂苷CK 30 mg/L+5-FU 25 mg/L)。采用MTT法检测各组细胞作用24、48、72 h后的细胞增殖抑制率,流式细胞术检测作用48 h后的细胞凋亡率,酶联免疫吸附法检测作用24、48、72、96 h后细胞中纤连蛋白表达,Western blot法检测作用48 h后细胞中波形蛋白、N-钙黏蛋白、E-钙黏蛋白、蛋白激酶(Akt)及磷酸化Akt(p-Akt)蛋白表达。结果:人参皂苷CK、5-FU及其二者联用对细胞的增殖均有抑制作用;与空白对照组比较,人参皂苷CK组、5-FU组和联用组各作用时间点的早期和晚期凋亡率、E-钙黏蛋白表达水平均明显升高(P0.05),纤连蛋白、波形蛋白、N-钙黏蛋白表达水平和p-Akt/Akt水平均明显降低(P0.05),其中联用组上述指标效果均优于人参皂苷CK组和5-FU组(P0.05)。结论:人参皂苷CK和5-FU均可抑制PANC-1细胞增殖、诱导细胞凋亡、抑制EMT,该作用可能与抑制磷脂酰肌醇3-激酶/Akt通路有关;且二者联用效果更好。
[Abstract]:Aim: to study the effects of ginsenoside CK combined with 5-fluorouracil (5-FU) on proliferation, apoptosis and (EMT) transformation in human pancreatic carcinoma PANC-1 cells. Methods: PANC-1 cells in logarithmic growth period were divided into blank control group, ginsenoside CK group (30 mg/L), 5-FU group (25 mg/L) and combination group (ginsenoside CK 30 mg/L 5-FU 25 mg/L). MTT assay was used to detect the cell proliferation inhibition rate after 24,48,72 h, flow cytometry was used to detect the apoptosis rate after 48 h, enzyme linked immunosorbent assay (Elisa) was used to detect the expression of fibronectin in the cells at 24, 48, 72, 96 h. The expression of vimentin, N-cadherin, E-cadherin, protein kinase (Akt) and phosphorylated Akt (p-Akt) protein were detected by Western blot assay. Results: ginsenoside CK,5-FU and its combination had inhibitory effect on cell proliferation. Compared with the blank control group, ginsenoside CK group, 5-FU group and combination group at the early and late stage of apoptosis, the expression of E-cadherin were significantly increased (P0.05), fibronectin, vimentin, and so on. The expression level of N-cadherin and the level of p-Akt/Akt decreased significantly (P0.05), and the effect of the above indexes in the combination group was better than that in the ginsenoside CK group and the 5-FU group (P0.05). Conclusion: both ginsenoside CK and 5-FU can inhibit the proliferation and induce apoptosis of PANC-1 cells, and the inhibition of EMT, may be related to the inhibition of phosphatidylinositol 3-kinase / Akt pathway.
【作者单位】：牡丹江医学院附属红旗医院消化科;牡丹江医学院附属红旗医院普外科;牡丹江医学院附属红旗医院泌尿外科;
【基金】：牡丹江市科学技术计划项目(No.Z2015s0055)
【分类号】：R96

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