1-脱氧野尻霉素对不同性别小鼠脂代谢产生影响的途径

发布时间：2019-05-10 00:29

【摘要】：以小鼠为实验动物,探究1-脱氧野尻霉素(DNJ)在动物体内促进脂质代谢的作用机制以及作用途径的性别差异。分别按照0.002、0.004和0.008 mg/(g·d)的剂量给正常小鼠灌胃DNJ 40 d后,用ELISA试剂盒测定试验组不同性别小鼠肝脏中的乙酰辅酶A羧化酶(ACC)活性、肉碱棕榈酰转移酶(CPT-1)活性,血清中的脂联素(ADP)含量、内脂素(visfation)含量、游离脂肪酸(FFA)含量。3个DNJ灌胃剂量组雌、雄小鼠的部分脂质代谢指标出现变化:雌鼠的ACC活性分别降低了5.85%、7.79%、10.08%,ADP含量分别升高了0.04%、0.25%、7.27%,FFA含量分别下降了9%、12%、19%;雄鼠的CPT-1活性分别上升了4.2%、8.1%、21.9%,ADP含量分别升高0.83%、4.17%、23.39%,内脂素含量分别升高1.4%、7.6%、10.2%,FFA含量分别下降了6.97%、7.81%、12.47%。研究结果提示DNJ对不同性别小鼠的脂代谢影响途径不尽一致:通过降低雌性小鼠的ACC活性和FFA含量以及提升ADP含量来抑制脂肪酸合成;通过提升雄鼠的CPT-1活性和ADP含量,降低FFA含量来促进脂肪酸分解。
[Abstract]:To explore the mechanism of 1-deoxynojirimycin (DNJ) promoting lipid metabolism in mice and the sex difference of its action pathway in mice, we studied the effects of 1-deoxynojirimycin on lipid metabolism in mice. After normal mice were given intragastrically at doses of 0.002, 0.004 and 0.008 mg/ (g 路d) for 40 days, the activity of acetylcoenzyme A Carboxylase (ACC) in the livers of mice of different genders was measured by ELISA kit. The activity of carnitine palmityltransferase (CPT-1), the content of adiponectin (ADP) in serum, the content of (visfation) in serum and the content of free fatty acid (FFA). Some lipid metabolism indexes of male mice changed: the activity of ACC in female mice decreased by 5.85%, 7.79%, 10.08%, the content of ACC increased by 0.04%, 0.25% and 7.27%, respectively, and the content of FFA decreased by 9%, respectively. 12%, 19%; The activity of CPT-1 increased by 4.2%, 8.1% and 21.9%, the content of CPT-1 increased by 0.83%, 4.17%, 23.39%, and the content of lipopolysaccharide increased by 1.4%, 7.6% and 10.2%, respectively. The content of FFA decreased by 6.97%, 7.81% and 12.47%, respectively. The results suggested that DNJ could inhibit fatty acid synthesis by decreasing the activity of ACC and the content of FFA and increasing the content of ADP in female mice. The fatty acid decomposition was promoted by increasing CPT-1 activity and ADP content and decreasing FFA content in male rats.
【作者单位】：西南大学食品科学学院重庆市农产品加工重点实验室;家蚕基因组生物学国家重点实验室;
【基金】：现代农业产业技术体系建设专项(No.CARS-22-0503) 公益性行业(农业)科研专项(No.201303053)
【分类号】：R965

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