液相色谱-四极杆飞行时间质谱检测染毒动物血浆中沙林和梭曼酪氨酸加合物
发布时间：2019-05-12 09:23
【摘要】：为了追溯性检测神经性毒剂沙林(GB)和梭曼(GD)暴露染毒,以染毒白蛋白上的特异性加合位点-411位酪氨酸加合物为重要生物标志物,建立了高效液相色谱-四极杆飞行时间质谱(LC/Q-TOF MS)分析动物血液中神经性毒剂GB和GD酪氨酸加合物的检测方法。在采用不同样品处理和仪器条件的基础上,确定了最佳实验条件。血液经离心后,取50μL分离的血浆加入到50μL 10 g/L链霉蛋白酶的NH4HCO3(50 mmol/L)水溶液中,于37℃孵育2.5 h,用0.5 m L 10 ku超滤管以16 000 r/min离心15min,LC/Q-TOF MS电喷雾正离子模式分析滤液。应用本方法进行GB半致死剂量(LD50)动物施毒实验,在施毒的动物血样中检测到膦酰化酪氨酸加合物,证明了该方法快速、简单、可靠。
[Abstract]:In order to detect the exposure of neurotoxic agents sarin (GB) and soman (GD) retroactively, the specific adduct site-411 tyrosine adduct on the exposed albumin was used as an important biomarker. A method for the determination of neurotoxic agents GB and GD in animal blood by high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (LC/Q-TOF MS) was established. On the basis of different sample treatment and instrument conditions, the optimum experimental conditions were determined. After centrifugation, 50 渭 L plasma was added to 50 渭 L 10 g 路L streptavidin NH4HCO3 (50 mmol/L) aqueous solution, incubated at 37 鈩，
本文编号：2475275
[Abstract]:In order to detect the exposure of neurotoxic agents sarin (GB) and soman (GD) retroactively, the specific adduct site-411 tyrosine adduct on the exposed albumin was used as an important biomarker. A method for the determination of neurotoxic agents GB and GD in animal blood by high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (LC/Q-TOF MS) was established. On the basis of different sample treatment and instrument conditions, the optimum experimental conditions were determined. After centrifugation, 50 渭 L plasma was added to 50 渭 L 10 g 路L streptavidin NH4HCO3 (50 mmol/L) aqueous solution, incubated at 37 鈩，
本文编号：2475275
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