硫酸依替米星注射液中有关物质测定方法的比较
发布时间：2019-05-30 00:04
【摘要】：目的系统比较电喷雾检测器、蒸发光散射检测器、脉冲安培生化学检测器3种方法测定依替米星注射液的有关物质,建立有关物质检测方法。方法建立的HPLC-CAD方法以0.2 mol·L~(-1)三氟乙酸-甲醇(95∶5)为流动相,流速为1.0 mL·min~(-1),柱温为30℃,雾化温度30℃,气压0.24 MPa,HPLC-ELSD和HPLC-PAD方法采用《中国药典》2015年版条件。结果 HPLC-CAD方法与HPLC-ELSD方法相比,分离度和灵敏度更高;与HPLC-PAD法比较,杂质测定结果更准确。结论建立的HPLC-CAD法分离能力强,检测出的杂质更多,可作为硫酸依替米星注射液有关物质测定的常规方法。
[Abstract]:Objective to systematically compare the determination of related substances in etimicin injection by electrospray detector, evaporative light scattering detector and pulse ampere chemical detector, and to establish a method for the determination of related substances. Methods the HPLC-CAD method was established with 0.2 mol 路L ~ (- 1) trifluoroacetic acid-methanol (95:5) as the mobile phase, the flow rate was 1.0 mL 路min ~ (- 1), the column temperature was 30 鈩，
本文编号：2488339
[Abstract]:Objective to systematically compare the determination of related substances in etimicin injection by electrospray detector, evaporative light scattering detector and pulse ampere chemical detector, and to establish a method for the determination of related substances. Methods the HPLC-CAD method was established with 0.2 mol 路L ~ (- 1) trifluoroacetic acid-methanol (95:5) as the mobile phase, the flow rate was 1.0 mL 路min ~ (- 1), the column temperature was 30 鈩，
本文编号：2488339
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