HPLC法测定人血浆中伏立康唑的浓度

发布时间：2019-05-30 05:34

【摘要】：目的:建立一种快速、灵敏、准确、稳定的测定人血浆中伏立康唑浓度的方法,用于伏立康唑临床治疗药物监测。方法:采用高效液相色谱(HPLC)-紫外检测法,以卡马西平为内标,乙腈蛋白沉淀法处理血浆样品。色谱柱为Phecda C18,流动相为20mmol/L磷酸二氢钾缓冲液(p H=6.0)-乙腈(50∶50,V/V),流速为1 ml/min,柱温为40℃,检测波长为255 nm,进样量为50μl。结果:伏立康唑和卡马西平的保留时间分别为8.34和6.24 min;血浆中伏立康唑线性范围为0.10~20.00μg/ml(r=0.999 5),定量下限为0.05μg/ml,日内、日间RSD分别为≤1.57%、1.45%,低、中、高梯度浓度提取回收率在81.40%~128.29%之间。结论:该方法操作简便,结果准确,适用于伏立康唑的临床治疗药物监测。
[Abstract]:Objective: to establish a rapid, sensitive, accurate and stable method for the determination of Voliconazole in human plasma for clinical drug monitoring. Methods: plasma samples were treated by high performance liquid chromatography (HPLC) and ultraviolet detection with carbamazepine as internal standard and acetonitrile protein precipitation method. The chromatographic column was Phecda C 18, the mobile phase was 20mmol/L potassium dihydrogen phosphate buffer (pH 6.0)-acetonitrile (50 脳 50, V 鈮，

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