超声介导载紫杉醇微泡对小鼠H22皮下移植瘤抑制作用的实验研究

发布时间：2019-06-01 06:57

【摘要】：目的探讨超声介导自制载紫杉醇微泡对小鼠H22皮下移植瘤的抑制效果。方法 (1)自制载紫杉醇微泡,并检测其一般特性。(2)建立小鼠H22皮下移植瘤模型,给予紫杉醇微泡+超声处理。绘制肿瘤生长曲线,计算各组抑瘤率,肿瘤行病理组织学检查,免疫组织化学法检测微血管密度(MVD),Western Blotting检测Bax、Bcl-2蛋白的表达。结果 (1)微泡呈圆形,表面光滑,平均粒径2.07μm,载药量10.13%,包封率89.58%。(2)紫杉醇微泡+超声组肿瘤生长明显减慢,肿瘤体积和质量明显下降(P0.05),肿瘤组织HE染色呈现不同程度变性坏死,MVD表达减少,Bax的表达上调,Bcl-2的表达下调(P0.05)。结论超声介导载紫杉醇微泡可明显抑制小鼠H22皮下移植瘤的生长,并可降低肿瘤组织MVD的表达,促进肿瘤细胞的凋亡。
[Abstract]:Objective to investigate the inhibitory effect of ultrasound-mediated paclitaxel microbubbles on subcutaneous transplantation of H22 in mice. Methods (1) paclitaxel microbubbles were made and their general characteristics were detected. (2) the mouse model of H22 subcutaneous transplantation tumor was established and treated with paclitaxel microbubble ultrasound. The tumor growth curve was drawn, the tumor inhibition rate was calculated, the tumor was examined by pathology, and the expression of Bax,Bcl-2 protein was detected by microvessel density (MVD), Western Blotting). Results (1) the microbubbles were round, the surface was smooth, the average particle size was 2.07 渭 m, the drug loading was 10.13%, and the entrapment efficiency was 89.58%. (2) the tumor growth in paclitaxel microbubble ultrasound group was significantly slower than that in paclitaxel microbubble ultrasound group. The tumor volume and mass decreased significantly (P 0.05), HE staining showed varying degrees of degeneration and necrosis, the expression of MVD decreased, the expression of Bax was up-regulated, and the expression of Bcl-2 was down-regulated (P 0.05). Conclusion Ultrasound-mediated paclitaxel-loaded microbubbles can significantly inhibit the growth of mouse H22 subcutaneous transplantation tumor, decrease the expression of MVD in tumor tissue and promote the apoptosis of tumor cells.
【作者单位】：三峡大学医学院肿瘤微环境与免疫治疗湖北省重点实验室;三峡大学第二临床医学院超声科;
【分类号】：R965

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