丙烯腈诱发活性氧生成对大鼠精子质量及超微结构的影响

发布时间：2019-06-03 21:30

【摘要】：目的探讨丙烯腈(ACN)诱导氧化应激对大鼠附睾精子质量与鞭毛超微结构的影响。方法 40只雄性SPF级健康SD大鼠按体重随机分为4组,染毒组以12.5、25和50 mg/kg ACN灌胃,对照组给予等体积玉米油。1次/d,6 d/周,连续13周。测精子活性氧(reactive oxygen species,ROS)、丙二醛(methane dicarboxylic aldehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、精子线粒体膜电位(mitochondrial membrane potential,MMP)和精子质量(0.86%生理盐水中游离精子),电子显微镜下观察精子鞭毛超微结构。结果 50 mg/kg组精子MMP较其他组均降低(P0.05)。50 mg/kg组精子ROS含量较其他组升高(P0.05)。染毒组SOD活力较对照组降低(P0.05)。精子鞭毛超微结构显示,25和50 mg/kg ACN组精子鞭毛外部致密纤维和线粒体结构有损伤。精子密度50 mg/kg组为(3.52±0.46)×10~6/ml,较对照组、12.5和25 mg/kg组[分别为(5.71±0.66)×10~6/ml、(4.95±1.33)×106/ml和(5.99±0.78)×106/ml]均降低(P0.05);25和50 mg/kg组精子活率分别为(62.18±3.4)%和(66.25±7.23)%,较对照组(83.22±13.86)%降低(P0.05)。d级精子率50 mg/kg组为(40.76±1.47)%,25 mg/kg组为(37.77±3.41)%,与对照组(27.77±4.85)%和12.5 mg/kg组(31.77±4.85)%比较均升高(P0.05)。精子平均路径速度对照组高于各ACN组,差异有统计学意义(P0.05)。直线速度50 mg/kg组较对照组降低(P0.05)。精子ROS含量与精子MMP呈负相关(r=-0.643,P=0.024),与SOD活力呈负相关(r=-0.713,P=0.009),与精子活力呈负相关(r=-0.629,P=0.028);与精子平均路径速度呈负相关(r=-0.839,P=0.001);与d级精子率呈正相关(r=0.699,P=0.011)。结论 ACN可致附睾内精子ROS含量增高,精子鞭毛内线粒体和外部致密纤维超微结构改变,而使精子活动能力和运动速度降低。
[Abstract]:Objective to investigate the effect of oxidative stress induced by acrylic (ACN) on sperm quality and flagellum ultrastructure in rat epididymis. Methods 40 male SPF healthy SD rats were randomly divided into 4 groups according to body weight. The exposed group was given 12.5, 25 and 50 mg/kg ACN intragastrically, while the control group was given equal volume corn oil once a day for 6 days for 13 weeks. Sperm reactive oxygen species (reactive oxygen species,ROS), malondialdehyde (methane dicarboxylic aldehyde,MDA), SOD (superoxide dismutase,SOD), sperm mitochondrial membrane potential (mitochondrial membrane potential,MMP) and sperm mass (0.86% saline free sperm) were measured. The ultrastructure of sperm flagellum was observed under electron microscope. Results the sperm MMP in 50 mg/kg group was lower than that in other groups (P 0.05). The sperm ROS content in 50 mg/kg group was higher than that in other groups (P 0.05). The activity of SOD in the exposed group was lower than that in the control group (P 0.05). The ultrastructure of sperm flagellum showed that the outer dense fibers and mitochondria of sperm flagellum were damaged in 25 and 50 mg/kg ACN groups. The sperm density in 50 mg/kg group was (3.52 卤0.46) 脳 10 鈮，

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