高效液相色谱-质谱联用法测定人血浆中左舒必利的浓度

发布时间：2019-06-04 12:06

【摘要】：目的建立测定人血浆中左舒必利的高效液相色谱-质谱联用(HPLC-MS/MS)方法。方法以硫必利为内标,血浆样品经甲醇沉淀蛋白提取分离,色谱柱为Xterra~汶RP18(4.6 mm×150 mm,5μm),流动相为乙腈-10 mmol·L~(-1)醋酸铵(含0.2%甲酸)水溶液(10:90),流速为1.0mL·min~(-1),柱温为30℃,选用电喷雾离子源(ESI)在正离子电离模式下,采用多反应监测(MRM)的质谱扫描方式。结果左舒必利标准曲线方程y=1.75×10~(-2)x+2.93×10~(-3)(n=3,r=0.999 3),线性范围1.00~600.00μg·L~(-1),定量下限为1.00μg·L~(-1),提取回收率在92.12%~98.53%,批内、批间RSD均小于4.61%。结论本方法专属性强,灵敏度高,操作简便、快速、准确,适用于血浆中左舒必利浓度的测定。
[Abstract]:Objective to establish a high performance liquid chromatography-mass spectrometry (HPLC-MS/MS) method for the determination of levosulpiride in human plasma. Methods using thiopyride as internal standard, plasma samples were extracted and separated by methanol precipitation protein on Xterra~ RP18 (4.6 mm 脳 150 mm, 5 渭 m). The mobile phase is acetonitrile-10 mmol 路L ~ (- 1) ammonium acetate (containing 0.2% formic acid) aqueous solution (10:90), the flow rate is 1.0mL 路min ~ (- 1), the column temperature is 30 鈩，

本文编号：2492710