高效液相色谱法检测干血滤纸片中卡马西平的浓度

发布时间：2019-06-20 04:42

【摘要】：目的:建立高效液相色谱法检测干血滤纸片中卡马西平血药浓度的方法。方法:将30μL含卡马西平的全血滴在滤纸上,室温下晾干至少3 h,待干透后从滤纸上打孔取下6 mm直径的血斑,放入1.5 mL普通离心管中,加入含有内标的甲醇溶液200μL,超声水浴10 min,涡旋1 min,15 000 r·min~(-1)离心5 min,取上清进样。色谱柱为AgilentTC-C_(18)柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(55∶45),内标为苯妥英,流速为1.0mL·min~(-1),检测波长为235 nm。结果:卡马西平血药浓度在1.0~32.0μg·mL~(-1)范围内与峰面积呈良好线性关系(r=0.999 0),方法回收率为101.3%~110.5%,提取回收率为59.8%~62.1%。结论:本方法操作简单、准确、可靠,适用于测定干血滤纸片中卡马西平的血药浓度。
[Abstract]:Objective: to establish a HPLC method for the determination of carbamazepine in dry blood filter paper. Methods: 30 渭 L whole blood droplets containing carbamazepine were dripped on the filter paper and dried at room temperature for at least 3 H. after drying through, the blood spots of 6 mm diameter were removed from the filter paper and put into 1.5 mL centrifuge tube. 200 渭 L methanol solution containing internal standard was added, and 1 min,15 000 r 路min ~ (- 1) centrifugation 5 min, with 10 min, vortex in ultrasonic water bath was used to take the sample. The chromatographic column was AgilentTC-C_ (18) column (250 mm 脳 4.6 mm, 5 渭 m), the mobile phase was methanol-water (55:45), the internal standard was phenytoin, the flow rate was 1.0mL 路min ~ (- 1), and the detection wavelength was 235 nm.. Results: there was a good linear relationship between carbamazepine and peak area in the range of 1.0 ~ 32.0 渭 g 路mL ~ (- 1) (r 鈮，

本文编号：2502940