辛伐他汀通过增加脂联素及其受体的表达改善高脂饮食引起的肾脏损伤

发布时间：2019-07-20 13:11

【摘要】：目的:探讨辛伐他汀保护高脂饮食致肾脏损伤的新机制。方法:雌性SD大鼠随机分为标准饮食组、高脂饮食组及辛伐他汀治疗组,高脂饮食组和辛伐他汀治疗组先予高脂饮食20周,后者在高脂饮食的同时予辛伐他汀~([10 mg/(kg·d)])灌胃治疗8周。分析大鼠血脂和肾脏的病理学变化、肾脏中脂联素、脂联素受体R1,R2,AMP依赖的蛋白激酶(AMPK),过氧化物酶体增殖物激活受体α(PPARα)、葡萄糖调节蛋白78(GRP78)和转录因子GADD153(CHOP)的表达。结果:高脂饮食组大鼠体质和血脂水平明显增高,脂联素及其受体表达明显下调,AMPKα磷酸化降低,PPARα表达降低,GRP78和CHOP基因表达水平均明显上调。辛伐他汀治疗组上述指标均出现明显改善。结论:辛伐他汀可能通过升高肾脏脂联素及其受体的表达,减少内质网应激来改善高脂诱导的大鼠肾脏损伤。
[Abstract]:Objective: to investigate the new mechanism of simvastatin in protecting kidney injury induced by high fat diet. Methods: female SD rats were randomly divided into standard diet group, high-fat diet group and simvastatin treatment group, high-fat diet group and simvastatin treatment group were given high-fat diet for 20 weeks, the latter was given simvastatin ~ (10 mg/ (kg 路d) intragastrically for 8 weeks. The pathological changes of blood lipid and kidney, the expression of adiponectin, adiponectin receptor R1, R2, (AMPK), peroxisome proliferator activated receptor 伪 (PPAR 伪), glucose regulated protein 78 (GRP78) and transcription factor GADD153 (CHOP) in kidney were analyzed. Results: in the high fat diet group, the levels of physique and blood lipid were significantly increased, the expression of adiponectin and its receptor was significantly down-regulated, the phosphorylation of AMPK 伪 was decreased, the expression of PAPAR 伪 was decreased, and the expression of GRP78 and CHOP genes was significantly up-regulated. The above indexes were significantly improved in simvastatin treatment group. Conclusion: simvastatin may improve renal injury induced by high fat by increasing the expression of adiponectin and its receptor in kidney and reducing endoplasmic reticulum stress.
【作者单位】：安徽医科大学生物化学教研室;安徽医科大学第一附属医院内分泌科;
【基金】：国家中医临床研究基地业务建设专项课题(编号:JDZX2015129)
【分类号】：R965

【参考文献】