鲫鱼活性多肽对小鼠体重、脂肪沉积和肝脏脂肪代谢酶活性的影响

发布时间：2019-07-25 16:22

【摘要】：为了探讨鲫鱼活性多肽的减肥作用机制,成功建立肥胖小鼠模型后,将72只雄性ICR小鼠随机分成6组,分别为空白组、模型组、鲫鱼多肽(CCP)低剂量组、中剂量组、高剂量组及阳性组。实验8周后,摘取肝脏和睾周脂肪并称重。通过测定各组小鼠血脂、抗氧化、脂肪代谢酶等相关指标,可知鲫鱼多肽能显著抑制小鼠体重的增加,其中高剂量鲫鱼多肽能显著降低小鼠体内甘油三酯(TG)、总胆固醇(TC)、游离脂肪酸(FFA)水平,显著降低肝脏中丙二醛(MDA)含量和脂肪酸合成酶(FAS)活性,显著提高谷胱甘肽过氧化酶(GSH-PX)水平、超氧化物歧化酶(SOD)活性、肉毒碱脂酰转移酶(CAT)和酰基辅酶A氧化酶(ACO)的活性。因此鲫鱼多肽可以通过改善机体氧化应激与炎症反应,减少脂肪合成,促进脂肪氧化,从而抑制体内脂肪沉积,达到减肥的目的。
[Abstract]:In order to explore the mechanism of weight loss of active polypeptide in crucian carp, 72 male ICR mice were randomly divided into 6 groups: blank group, model group, crucian carp polypeptide (CCP) low dose group, middle dose group, high dose group and positive group. After 8 weeks of the experiment, the liver and peri-testicular fat were removed and weighed. The results showed that crucian carp polypeptide could significantly inhibit the weight gain of mice, in which high dose crucian carp polypeptide could significantly decrease the level of TG (TG), total cholesterol (TC), free fatty acid (FFA) in mice, and significantly decrease the content of malondialdehyde (MDA) (MDA) and the activity of fatty acid synthase (FAS) in liver. The level of glutathione peroxide (GSH-PX), the activity of SOD (SOD), the activity of carnitine lipidyltransferase (CAT) and acyl coenzyme A oxidase (ACO) were significantly increased. Therefore, crucian carp polypeptide can reduce fat synthesis and promote fat oxidation by improving oxidative stress and inflammatory reaction, so as to inhibit fat deposition in vivo and achieve the purpose of weight loss.
【作者单位】：河北农业大学海洋学院;天津科技大学食品工程与生物技术学院;
【基金】：河北省教育厅青年基金项目(QN2015245)
【分类号】：R965

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