流动注射化学发光法测定奈替米星

发布时间：2019-07-28 16:17

【摘要】：目的建立流动注射化学发光法测定血浆中奈替米星的方法。方法在碱性介质中,奈替米星对铁氰化钾与鲁米诺化学发光体系具有明显的增敏作用,据此建立了一种测定奈替米星的流动注射化学发光新方法,对分析条件进行优化。通过方法学评价及对血浆奈替米星的测定,观察建立方法的性能。结果优化后的最佳检测条件是:鲁米诺浓度为10μmol/L,铁氰化钾的浓度为30μmol/L,Na OH的浓度为0.10mol/L,采样时间为5s,泵速为30r/min,负高压为630V。在确定的最佳实验条件下,化学发光增加强度与奈替米星的浓度在1.0×10~(-6)~5.0×10~(-5)g/m L范围内呈良好的线性关系,检测限为9.2×10~(-7)g/m L。对含3.6×10~(-6)g/m L奈替米星的溶液平行测定11次,测定结果的相对标准偏差为2.3%。结论本方法分析速度快、灵敏度高、分析成本低,可用于血浆中奈替米星的测定。
[Abstract]:Objective to establish a flow injection chemiluminescence method for the determination of netilmicin in plasma. Methods in alkaline medium, netilmicin had obvious sensitizing effect on potassium ferricyanide and luminol chemiluminescence system. A new flow injection chemiluminescence method for the determination of netilmicin was established and the analytical conditions were optimized. The performance of the established method was observed by methodological evaluation and determination of netilmicin in plasma. Results the optimum detection conditions were as follows: the concentration of luminol was 10 渭 mol / L, the concentration of potassium ferricyanide was 30 渭 mol / L, the concentration of Na OH was 0.10 mol 路L, the sampling time was 5 s, the pump speed was 30 r / min, and the negative pressure was 630 V. Under the optimum experimental conditions, there is a good linear relationship between the intensity of chemiluminescence and the concentration of netilmicin in the range of 1.0 脳 10 ~ (- 6) ~ 5.0 脳 10 ~ (- 5) g 路mL, and the detection limit is 9.2 脳 10 ~ (- 7) g 路mL ~ (- 1). The relative standard deviation of the solution containing 3.6 脳 10 ~ (- 6) g 脳 10 ~ (- 6) g / L netilmicin was 2.3%. Conclusion this method has the advantages of fast analysis speed, high sensitivity and low analysis cost. It can be used for the determination of netilmicin in plasma.
【作者单位】：重庆医科大学检验医学院临床检验诊断学教育部重点实验室;
【分类号】：R927.2

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