体外和体内磷酸肌酸可降低血糖水平，改善肝细胞内质网应激的糖尿病小鼠

发布时间：2021-08-12 04:01

　　背景肝细胞受损可增加糖尿病（Diabetes mellituspatients,DM）患者发病率和死亡率。已公认,高血糖是DM患者肝损害发病的主要原因,与DM相关的肝细胞损害是DM患者常见的并发症。磷酸肌酸（Phosphocreatine,PCr）分子作为一种可迅速动员、回收ATP,对脑和骨骼肌的能量储备,可作为细胞能量储备形式,它具有多种活性。然而,PCr是否具有保护DM相关肝损伤的作用尚未见报道。本研究旨在评价PCr是否能保护DM并发的肝损伤,并探讨其在体内外作用机制。材料与方法体外研究:丙酮醛（methylglyoxal,MGO）,是葡萄糖的活性代谢产物,与高血糖可引起DM相关的肝细胞损伤,且与肝细胞损害进展关系密切。Hep G2细胞培养及细胞进行预处理MGO两小时,然后,分别用不同浓度的PCR处理细胞（5m M,10m M,20m M）。采用MTT法研究胞形态学、细胞毒性、细胞凋亡;流式细胞仪Annexin V-FITC、Calcien法分别检测细胞凋亡、增殖试验。通过Western blotting检测凋亡蛋白Bax、Bcl-2和Caspase-3、Caspase-9蛋白表达... 

【文章来源】：大连医科大学辽宁省

【文章页数】：71 页

【学位级别】：硕士

【文章目录】：
ABSTRACT
摘要
Abbreviations list
1. INTRODUCTION
    1.1 Study hypothesis and objectives
    1.2 Biomarkers
2. MATERIALS AND METHODS
    2.1 Materials
    2.2 METHODS
        2.2.1 Cell culture and reagents
        2.2.2 MTT cells toxicity
        2.2.3 Cells Morphology Assay
        2.2.4 Calcien AM Cells Proliferation Assay
        2.2.5 Wound Healing Assay
        2.2.6 Western Blotting Analysis
        2.2.7 Animal model
        2.2.8A nimal Experimental Design and Follow up
3. Statistical Analysis
4. RESULTS
    4.1 The Effect of PCr on Cells Morphology and Structure
    4.2 PCr enhances cells proliferation and decrease cells apoptosis
    4.3 PCr enhances wound healing process
    4.4 PCrameliorates Hepatic Endoplasmic Reticulum Stress markers
    4.5 Effect of PCr on blood glucose level and body mass
    4.6 Effect of PCr on liver biochemical biomarkers
    4.7 Effect of PCr on liver tissues histology
5. DISCUSSION
CONCLUSIONS
Acknowledgements and Research fund
REFERENCES
Literature Review Diabetes mellitus (DM) and Liver Injury
    1.Background
    2.ETYMOLOGY OF DIABETES MELLITUS
    3.Epidemiology
    4.Pathophysiology of type 1 Diabetes Mellitus
        4.1 Role of Insulin in Diabetes Mellitus
        4.2 Factors Causing Diabetes
    5.Complications associated with DM
        5.1 Diabetic retinopathy
        5.2 Glaucoma
        5.3 Cardiovascular diseases associated with diabetes
        5.4 Diabetic nephropathy
        5.5 Diabetic neuropathy
        5.6 Liver injury associated with DM
    6.Biomarkers
    Conclusions and Perspectives
    REFERENCES
APPENDIX-1 Blood glucose and body weight data
PUBLISHED RESEARCH
ACKNOWLEDGEMENT



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