理想的电生理学实验平台:急性分离的成年比格犬迷走神经节神经元（英文）
发布时间：2022-10-21 09:53
目的建立急性分离成年犬结状神经节神经元（VGN）电生理学研究平台。方法通过急性分离和培养雌雄成年比格犬VGN,利用全细胞膜片钳技术分别记录动作电位,电压依赖性的外向钾电流（IK）和超极化激活的阳离子电流（Ih）。结果动作电位波形分析结果显示:（1）雌性比格犬VGN不仅可以记录到传统分类中的髓鞘化A型和非髓鞘化C型神经元,还可以记录到雌性特异分布的Ah型神经元;（2）步阶电流除极在诱发相同持续放电频率时,A型所需要的刺激强度最小,Ah型所需要的电流强度与A型近似或轻微增加,而C型需要的刺激强度最大;（3）河豚毒素显著降低Ah型神经元的除极速率和触及阈值;蝎毒素可显著增加A-型的神经兴奋性;（4）所有受试神经元均表达IK和Ih,且2种电流密度均呈现A型＞Ah型＞C型;（5）尽管雌雄VGN分布的传入纤维种类有别,已知的A和C型VGN放电差异性并未在本研究中探讨。结论成年比格犬VGN可被成功急性分离,并用于动作电位、IK和Ih的记录;分类和电生理学特性足以完成药理...
【文章页数】：12 页
【文章目录】：
1 MATERIALS AND METHODS
1.1 Animals,reagents and instruments
1.2 Whole-cell patch-clamp experiments and neuron identification
1.3 Isolation of vagal ganglion neurons
1.4 Extracellular solution of AP,repetitive discharge,hyperpolarization-mediated inward currents(Ih) and whole-cell K+currents (Ik)recordings
1.5 Afferent fiber classification
1.6 Statistical analysis
2 RESULTS
2.1 Isolated neurons
2.2 Action potential discharge characteristics
2.3 Repetitive discharge profiles
2.4 Functional expression of voltage-gated Na+channels (TTX-S:Nav1.7,TTX-R:Nav1.8)
2.5 Functional expression of large conduc-tance Ca2+-activated K+(KCa1.1) channel
2.6 Functional expression of Ihand IK
3 DISCUSSION
【参考文献】：
期刊论文
[1]Therapeutic Efficacy of Neurostimulation for Depression:Techniques, Current Modalities, and Future Challenges[J]. Hafsah Akhtar,Faiza Bukhari,Misbah Nazir,Muhammad Nabeel Anwar,Adeeb Shahzad. Neuroscience Bulletin. 2016(01)
[2]用膜片箝技术比较大鼠分离细胞和完整结状神经节的动作电位(英文)[J]. LIBai-Yan ,JohnHSCHILD. Acta Pharmacologica Sinica. 2002(06)
本文编号：3695412
【文章页数】：12 页
【文章目录】：
1 MATERIALS AND METHODS
1.1 Animals,reagents and instruments
1.2 Whole-cell patch-clamp experiments and neuron identification
1.3 Isolation of vagal ganglion neurons
1.4 Extracellular solution of AP,repetitive discharge,hyperpolarization-mediated inward currents(Ih) and whole-cell K+currents (Ik)recordings
1.5 Afferent fiber classification
1.6 Statistical analysis
2 RESULTS
2.1 Isolated neurons
2.2 Action potential discharge characteristics
2.3 Repetitive discharge profiles
2.4 Functional expression of voltage-gated Na+channels (TTX-S:Nav1.7,TTX-R:Nav1.8)
2.5 Functional expression of large conduc-tance Ca2+-activated K+(KCa1.1) channel
2.6 Functional expression of Ihand IK
3 DISCUSSION
【参考文献】：
期刊论文
[1]Therapeutic Efficacy of Neurostimulation for Depression:Techniques, Current Modalities, and Future Challenges[J]. Hafsah Akhtar,Faiza Bukhari,Misbah Nazir,Muhammad Nabeel Anwar,Adeeb Shahzad. Neuroscience Bulletin. 2016(01)
[2]用膜片箝技术比较大鼠分离细胞和完整结状神经节的动作电位(英文)[J]. LIBai-Yan ,JohnHSCHILD. Acta Pharmacologica Sinica. 2002(06)
本文编号：3695412
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