TNFα和IL-6体外对颗粒物的反应:随颗粒物大

发布时间：2018-01-03 06:04

  本文关键词：TNFα和IL-6体外对颗粒物的反应:随颗粒物大小、季节、多环芳烃和土壤含量的变化　出处：《环境与职业医学》2016年12期 　论文类型：期刊论文

  更多相关文章： 多环芳烃 内毒素检测 采集样 流行病学研究 细胞因子 主成分分析 肿瘤坏死因子α 城市地区 苯并 元素浓度

【摘要】：[背景]颗粒物(PM)与人类健康之间的关联随着季节变化而不同,这种现象可能与颗粒物的成分以及毒性相关的季节性交互作用有关。[目的]检测PM成分的季节性变化,并使用多个PM样本评估PM的体外促炎症可能性。[方法]以5个污染源不同的城市地区为采集地点,每周采集样本,在多雨温暖季节和干燥寒冷季节累积采集90份PM_(10)和PM_(2.5)样本。对其中检测到的元素、多环芳烃(PAHs)和内毒素进行主成分分析(PCA)。在人单核细胞(THP-1)中检测PM诱导肿瘤坏死因子α(TNFα)和白介素6(IL-6)分泌的可能性,并用组分评分建立促炎症反应的模型。[结果]PM的成分随其大小和季节而变化。PCA确定了PM中两种随季节变化的主要组分。燃烧相关的成分(如钒、苯并(a)芘、苯并(a)蒽)组成组分1(C_1),土壤相关的成分(如内毒素、硅、铝)组成组分2(C_2)。多雨温暖季节采集的PM中C_2含量较高,而干燥寒冷季节采集的PM(尤其是PM_(2.5))中C_1含量较高。细胞因子生成水平与PM_(10)和C_2(多雨温暖季节)正相关,但与PM_(2.5)和C_1(干燥寒冷季节)负相关。C_2含量较高的PM暴露水平越高,TNFα分泌越多;样本含有≥0.1%的C_1相关PAHs时,无论样本中C_2含量高低,暴露于该样本后TNFα对PM的反应性分泌都会降低。IL-6检测结果提示PM成分和颗粒大小之间存在复杂的交互作用。[结论]TNFα分泌具有季节性并与PM大小相关,PM中的土壤含量及PAH含量的变化是其根本原因。这些结果提示,流行病学研究中一些健康结局与PM之间关联的季节性差异能用PM组分的混合物来解释。
[Abstract]:[background] particles (PM) and the relationship between human health with the seasonal changes and different, this phenomenon may be related to particle composition and toxicity related to seasonal interaction between seasonal changes related to the composition. To detect PM, and use multiple PM samples to assess PM in vivo proinflammatory possibility. Methods in 5 different sources of pollution in the city area for the collection of locations, weekly samples collected during the rainy season and dry, warm cold season cumulative acquisition of 90 PM_ (10) and PM_ (2.5) samples. The detected elements, polycyclic aromatic hydrocarbons (PAHs) and endotoxin were analyzed by principal component analysis (PCA). Human mononuclear cells (THP-1) in the detection of PM induced tumor necrosis factor alpha (TNF alpha) and interleukin 6 (IL-6) secretion and the possibility of score model. The results of proinflammatory]PM components with their size and seasonal changes of.PCA and identified two PM with 瀛ｈ妭鍙樺寲鐨勪富瑕佺粍鍒，

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