深圳市某区淡水产品中香港海鸥菌的检测及耐药特征分析

发布时间：2018-01-14 05:33

本文关键词：深圳市某区淡水产品中香港海鸥菌的检测及耐药特征分析　出处：《中华疾病控制杂志》2016年08期 　论文类型：期刊论文

【摘要】：目的了解深圳市南山区淡水产品中香港海鸥菌的污染情况,并分析其耐药特征。方法于2015年4~9月采购该区1家水产批发市场、1家超市和3家农贸市场淡水产品样本共计347份,检样接种改良头孢哌酮麦康凯琼脂(cefoperazone macconkey agar,CMA)培养分离可疑菌株,经革兰染色、生化试验及16S rRNA扩增测序确认;阳性菌株用纸片扩散法试验19种抗生素耐药性。结果在347份淡水产品中检出香港海鸥菌108份,阳性率31.12%,其中草鱼阳性率20.44%,虎纹蛙阳性率50.82%。蛙株和鱼株药敏结果比较显示:鱼来源株对一、三类头孢菌素类抗生素耐药率高,蛙来源株对四环素类、喹诺酮类和磺胺类耐药率高。在38株多重耐药香港海鸥菌中,9株鱼分离株、29株蛙分离株分别表现为6种和20种耐药表型。结论深圳市该区淡水产品中存在不同程度的香港海鸥菌的污染且蛙来源的香港海鸥菌耐药模式更加复杂。
[Abstract]:Objective to investigate the contamination of Hong Kong seagull in fresh water products from Nanshan District, Shenzhen City, and to analyze its drug resistance characteristics. Methods A wholesale aquatic market was purchased from 2015 to September in this area. A total of 347 samples of fresh water products from one supermarket and three farmers' markets were inoculated with improved cefoperazone macconkey agar. The suspicious strains were isolated by CMA and confirmed by Gram staining, biochemical test and 16s rRNA amplification and sequencing. Results 108 strains of Hong Kong seagulls were detected in 347 fresh water products, and the positive rate was 31.12%, of which grass carp positive rate was 20.44%. The positive rate of Rana rubra was 50.82. The results of drug sensitivity of frog strain and fish strain showed that the resistance rate of fish strain to one or three kinds of cephalosporins antibiotics was high, and frog strain was to tetracycline. The resistance rates of quinolones and sulfanilamides were high. Among 38 strains of multidrug resistant Hong Kong seagull strains, 9 were fish isolates. Conclusion there are different degrees of contamination of Hong Kong seagull bacteria in fresh water products in Shenzhen City, and the drug resistance patterns of Hong Kong gulls from frog origin are more complex.
【作者单位】：南方医科大学公共卫生与热带医学学院流行病学系;深圳市疾病预防控制中心微生物检验科;南方医科大学南方医院感染内科;
【基金】：国家自然科学基金(81373052)
【分类号】：R155.5
【正文快照】： (Chin J Dis Control Prev 2016,20(8):764-767)香港海鸥菌(laribacter hongkongensis,LH)是新发现的可引起人类胃肠炎的病原菌之一,与产肠毒素脆弱类杆菌和产酸克雷伯菌一起被称为“21世纪新发现的三大腹泻致病菌”[1]。因首次分离于香港且镜检呈海鸥形或螺旋形杆菌而得名[2]

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