SPE-HPLC法检测早胜牛肉中土霉素、四环素和金霉素的残留

发布时间：2018-01-14 06:13

  本文关键词：SPE-HPLC法检测早胜牛肉中土霉素、四环素和金霉素的残留　出处：《国际药学研究杂志》2016年02期 　论文类型：期刊论文

  更多相关文章： 固相萃取-高效液相色谱法 早胜牛肉 土霉素 四环素 金霉素

【摘要】：目的建立同时测定早胜牛肉中残留的土霉素、四环素和金霉素固相萃取-高效液相色谱方法。方法以Na2EDTA-Mcllvaine缓冲溶液(p H4.0)为样品提取液,15%三氯乙酸为蛋白沉淀剂,固相萃取柱净化富集目标物,草酸0.01mol/L/乙腈/甲醇溶液为流动相,紫外检测器350 nm处检测,外标法进行定量。结果在优化前处理和分析条件下,3种抗生素在质量浓度范围内线性关系良好,相关系数r20.998,土霉素、四环素、金霉素的检出限分别为18、15和30μg/kg,样品加标平均回收率为76.10%~95.22%,相对标准偏差10%。结论方法的灵敏度和精密度较高,且重复性好,适用于牛肉样品中土霉素、四环素和金霉素残留的检测。
[Abstract]:Objective to establish a simultaneous determination of oxytetracycline in Zaosheng beef. Tetracycline and chlortetracycline were extracted by high performance liquid chromatography (HPLC). Methods the Na2EDTA-Mcllvaine buffer solution was used as the sample extraction solution. 15% trichloroacetic acid was used as protein precipitating agent, solid phase extraction column was used to purify and enrich the target, oxalic acid 0.01mol / L / acetonitrile / methanol solution was used as mobile phase, and UV detector was 350nm. Results under the optimized conditions of pretreatment and analysis, there was a good linear relationship between the three antibiotics in the range of mass concentration, the correlation coefficient was r20.998, oxytetracycline and tetracycline. The detection limits of chlortetracycline were 18 渭 g / kg and 30 渭 g / kg, respectively. The average recoveries of chlortetracycline were 76.10% and 95.22% respectively. Conclusion the method has high sensitivity, precision and reproducibility. It is suitable for the determination of oxytetracycline, chlortetracycline and chlortetracycline residues in beef samples.
【作者单位】： 西北民族大学生命科学与工程学院;甘肃省动物细胞工程技术研究中心;
【基金】：新丝路经济带清真食品生产技术升级与品牌创新模式研究及应用示范(2015BAD29B05) 教育部创新团队发展计划(IRT13091)
【分类号】：R155.55
【正文快照】： 四环素族抗生素是一类由放线菌属产生的广谱抗菌剂,土霉素(oxytetracycline,OTC)、四环素(tetrarcycline,TC)和金霉素(chlortetracycline,CTC)等是其典型代表。四环素族抗生素对革兰阳性菌和阴性菌、螺旋体、病毒等有较好的抑制作用,且具有价格低廉等优点,常用于畜禽细菌性疾，

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