双歧醋对高脂饮食大鼠预防性减肥和降脂的实验研究

发布时间：2018-01-26 21:37

本文关键词： 双歧醋大鼠减肥高脂血症机制　出处：《重庆医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：研究双歧醋对高脂饮食大鼠预防性减肥和降脂的作用，以及对机制的探讨。方法： 1.双歧醋对高脂饮食大鼠预防性减肥的实验研究：将SD大鼠随机分成双歧醋低、中、高剂量组、市售醋组、肥胖模型组以及基础对照组，灌胃法喂饲大鼠，除基础对照组喂食基础饲料外，其余组均喂食高营养饲料。测定指标包括大鼠体重、体脂、Lee′s指数、脂肪细胞直径、血瘦素、血胰岛素。 2.双歧醋对高脂饮食大鼠血脂的影响：将SD大鼠随机分成双歧醋低、中、高剂量组、市售醋组、高脂模型组以及基础对照组，灌胃法喂饲大鼠，除基础对照组喂食基础饲料外，其余组均喂食高脂营养饲料。测定指标包括大鼠体重、肝指数、体脂指数、病理学观察肝脏脂肪变性。 3.双歧醋对高脂饮食大鼠预防性降脂的机制探讨：将SD大鼠随机分成双歧醋低、中、高剂量组、市售醋组、高脂模型组以及基础对照组，灌胃法喂饲大鼠，除基础对照组喂食基础饲料外，其余组均喂食高脂营养饲料。测定指标包括大鼠肝脏脂质、肝脏脂肪变性、低密度脂蛋白受体(LDLR)的免疫组化分析、羟甲基戊二酸单酰CoA（HMGCoA）还原酶和LDLR的RT-PCR分析。结果： 1.与肥胖模型组比较，双歧醋各组大鼠的体重、体脂、Lee′s指数、脂肪细胞直径、血瘦素、血胰岛素均有明显好转，特别是血瘦素和血胰岛素水平。 2.与高脂模型组比较，双歧醋各组的大鼠体重、肝指数、体脂指数、肝脏脂肪变性程度均有明显好转，，特别是甘油三酯(TG)和高密度脂蛋白胆固醇(HDL-C)水平。 3.双歧醋对HMGCoA还原酶表达有抑制作用，但可以上调LDLR的基因和蛋白质表达。结论：双歧醋在预防高脂饮食大鼠肥胖和血脂升高方面有一定的作用，并有剂量效应关系。
[Abstract]:Objective: To study the effect of bifidobacterium vinegar on preventive weight loss and lipid lowering in rats with high fat diet and to explore the mechanism. Methods: 1. Experimental study of Bifidobacterium Acetate on Preventive weight loss in Rats with High Fat Diet: SD rats were randomly divided into three groups: low, medium, high dose, market vinegar, obesity model and basic control group. The rats were fed with the basic diet except the basic control group and the other groups were fed with the high nutrition diet. The parameters included body weight, body fat Leehs index, adipocyte diameter and blood leptin. Blood insulin. 2. Effects of Bifidobacterium Acetate on Blood Lipid in Rats with High Fat Diet: SD rats were randomly divided into three groups: low, medium, high dose, commercial vinegar, hyperlipidemia model and basic control group. All of the control groups were fed with high fat nutritional diet except the basic control group, including body weight, liver index, body fat index and pathological observation of hepatic steatosis. 3. Mechanism of Bifidobacterium Acetate on preventing Lipid lowering in Rats with High Fat Diet: SD rats were randomly divided into three groups: low, medium, high dose, market vinegar, hyperlipidemia model and basic control group. The rats were fed by gavage, except the basic diet for the basic control group, and the other groups were fed with the high fat nutritive diet, including the lipids of the liver and fatty degeneration of the liver. Immunohistochemical analysis of low density lipoprotein receptor (LDLR), RT-PCR analysis of hydroxymethyl glutaric acid monoyl CoA (HMG CoA) reductase and LDLR. Results: 1. Compared with the obese model group, the body weight, body fat Leehs index, adipocyte diameter, blood leptin and serum insulin of the rats in the bifidobacterium vinegar group were significantly improved. In particular, serum leptin and insulin levels. 2.Compared with the hyperlipidemia model group, the body weight, liver index, body fat index and degree of hepatic steatosis of the rats in the bifidobacterium vinegar group were obviously improved. In particular, the levels of triglyceride (TG) and high density lipoprotein cholesterol (HDL-C). 3. Bifid vinegar can inhibit the expression of HMGCoA reductase, but can up-regulate the expression of LDLR gene and protein. Conclusion: Bifidobacterium vinegar plays a certain role in preventing obesity and hyperlipidemia in rats with high fat diet and has a dose-effect relationship.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R151

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