梯度剂量放射对兔髁状突组织学结构影响的研究

发布时间：2018-02-11 02:52

本文关键词： 放射疗法放射治疗剂量下颌骨髁状突组织学组织学比较兔　出处：《福建医科大学学报》2015年05期 　论文类型：期刊论文

【摘要】：目的采用梯度剂量放射线照射兔颞下颌关节,动态观察研究兔髁状突的组织学改变。方法取新西兰兔63只,分为未放疗组和放疗组,未放疗组作为空白对照,不做任何处理,用于收集正常颞下颌关节资料;放疗组兔随机分为A~F 6个剂量组,采用梯度剂量行颞下颌关节区放射,剂量分别为10.8,16.2,21.6,27.0,32.4,37.8Gy,于放疗后1,3,6月每组各处死3只,完整切取颞下颌关节,H-E染色后光镜下观察兔髁状突组织学改变。结果放疗后髁状突软骨纤维层疏松、玻璃样变性,纤维间的间隙增宽,增殖层细胞变形、排列紊乱,肥大层、增殖层和钙化软骨层均有不同程度的变薄,层次不明显,细胞数量减少;放疗后关节结构的组织学改变的严重程度随着放疗剂量的增大及放疗后时间的延长而越趋严重,F组的关节结构破坏最为严重,且6月组较1,3月组严重。结论放疗在早期导致颞下颌关节在组织学上出现一系列退行性变,且这种改变随着放疗剂量和放疗后时间的延长而加重,但期间并未出现关节强直的组织学改变。
[Abstract]:Objective to observe the histological changes of condyle in rabbits with gradient dose radiation. Methods Sixty-three New Zealand rabbits were divided into two groups: the non-radiotherapy group and the radiotherapy group. The rabbits in the radiotherapy group were randomly divided into 6 dose groups of Agna F, with gradient doses of 10.8 ~ 16.21.62 ~ 27.0 ~ 37.8 Gy, respectively, and 3 rabbits died in each group on June, 1 day after radiotherapy, and 3 rabbits in each group were killed in each group. Results the fibrous layer of condylar cartilage was loose, vitreous degeneration, interfibrous space widened, proliferative layer cells deformed, disordered, hypertrophic layer, hypertrophic layer, and hypertrophic layer of condylar chondrocytes were observed under light microscope after complete resection of temporomandibular joint with H-E staining. The proliferation layer and calcified cartilage layer were thinned to some extent, the layers were not obvious, and the number of cells decreased. With the increase of radiation dose and the prolongation of time after radiotherapy, the severity of histological changes of articular structure in group F was more serious than that in group F. Conclusion radiotherapy may cause a series of histologically degenerative changes of TMJ in the early stage of radiotherapy, and this change is aggravated with the prolongation of radiotherapy dose and time after radiotherapy. But there was no histological change in ankylosis during the period.
【作者单位】：福建医科大学附属第一医院口腔颌面外科;
【分类号】：R144.1

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