亚砷酸钠对雄性大鼠炎症因子和氧化应激的影响

发布时间：2018-02-11 06:05

本文关键词： 砷亚砷酸钠炎症因子氧化应激　出处：《环境与职业医学》2017年06期 　论文类型：期刊论文

【摘要】：[目的]探讨亚砷酸钠对雄性大鼠血清中炎症因子和氧化应激水平的影响。[方法]将32只健康无特定病原级雄性SD大鼠按体重随机分为4组,分别为对照组(超纯水)和低(10μg/L)、中(100μg/L)、高(1 000μg/L)剂量亚砷酸钠溶液染毒组。每组8只,自由饮水染毒28 d。采用酶联免疫吸附试验测定大鼠血清中粒细胞-巨噬细胞集落刺激因子(GM-CSF)、白细胞介素-10(IL-10)、还原型烟酰胺腺嘌吟二核苷酸磷酸(NADPH)氧化酶、活性氧簇酶(ROS)、总抗氧化能力和一氧化氮(NO)水平。[结果]亚砷酸钠染毒组大鼠血清GM-CSF表达量显著高于对照组(P0.05),且1 000μg/L组的GM-CSF水平最高;抑炎因子IL-10含量均低于对照组(P0.05);1 000μg/L剂量组ROS表达水平高于对照组(P0.05);与对照组相比,各染毒组NO表达量升高(P0.05),且存在剂量-反应关系。[结论]亚砷酸钠进入雄性大鼠机体后产生炎症损伤和氧化应激损伤,损伤机制可能与NO增多、IL-10降低有关。
[Abstract]:[objective] to investigate the effect of sodium arsenite on the levels of inflammatory factors and oxidative stress in serum of male rats. [methods] Thirty-two healthy male SD rats without specific pathogen were randomly divided into 4 groups according to their body weight. The control group (ultrapure water) and the control group (10 渭 g / L), the control group (100 渭 g / L), and the high dose (1 000 渭 g / L) sodium arsenite solution were treated with sodium arsenite solution (8 rats in each group). The enzyme linked immunosorbent assay (Elisa) was used to determine granulocyte-macrophage colony stimulating factor (GM-CSFN), interleukin-10 (IL-10) IL-10, and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase in serum of rats exposed to free drinking water for 28 days. [results] the expression of GM-CSF in serum of rats exposed to sodium arsenite was significantly higher than that of control group (P 0.05), and the GM-CSF level of 1 000 渭 g / L group was the highest. The level of ROS expression in the control group was lower than that in the control group (P 0.05 渭 g / L), and the level of ROS in the control group was higher than that in the control group (P 0.05 渭 g / L). There was a dose-response relationship between no expression and no expression in each group. [conclusion] Sodium arsenite induced inflammatory injury and oxidative stress injury after entering male rats, and the mechanism may be related to the decrease of IL-10.
【作者单位】：包头医学院公共卫生学院;
【基金】：国家自然科学基金项目(编号:81660532) 内蒙古科技计划项目(编号:201502080)
【分类号】：R114

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