遗传进化分析在广东省一起HCV“暴发流行”事件中的应用

发布时间：2018-03-24 14:45

本文选题：丙型肝炎病毒　切入点：暴发　出处：《实用医学杂志》2017年07期

【摘要】：目的:通过对广东省一起丙型肝炎病毒(HCV)"暴发流行"事件进行遗传进化分析来了解此次事件的起源时间和传播源头。方法:首先对"暴发流行"事件调查中的192例血清样本作为研究组进行基因分型,得到2a亚型和6a亚型,选取相应基因亚型的对照组样本,采用生物信息学相关软件进行遗传进化分析,通过构建的时间尺度树推断此次HCV可能的起源时间及传播源头。结果:此次"暴发流行"事件的192例患者中,66例为2a亚型,119例为6a亚型,未发现其它的基因亚型。对2a亚型数据集以及6a数据集分别进行遗传进化分析提示两者都主要起源于2~5年前,均符合医源性感染的特点。结论:此次广东省HCV"暴发流行"事件中HCV主要基因型为2a和6a,主要起源于2~5年前,符合医源性传播特点,随着时间的延长积累了众多病例导致"暴发"。
[Abstract]:Objective: to investigate the origin time and transmission source of an outbreak of hepatitis C virus (HCV) in Guangdong Province by genetic and evolutionary analysis. Methods: firstly, 192 cases of "outbreak" were investigated. Serum samples were used as study group for genotyping. 2a subtype and 6a subtype were obtained. The control group samples of the corresponding gene subtypes were selected, and the genetic evolution analysis was carried out by bioinformatics software. According to the time scale tree constructed, the possible origin and source of HCV transmission were inferred. Results: among the 192 patients with this outbreak, 66 cases were subtype 2a and 119 cases were subtype 6a. No other gene subtypes were found. Genetic evolution analysis of 2a subtype data set and 6a data set showed that both of them mainly originated from 2 ~ 5 years ago. Conclusion: in this outbreak of HCV in Guangdong Province, the main genotypes of HCV were 2a and 6a, which were mainly originated from 2 ~ 5 years ago and accorded with the characteristics of iatrogenic transmission. Over time, the accumulation of many cases led to an "outbreak".
【作者单位】：中山大学附属第三医院感染科;中山大学附属第三医院肝移植科、外科重症医学科;
【基金】：正大天晴Bridge专项经费资助(编号:20151211)
【分类号】：R181.3;R512.63

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