邻苯二甲酸单乙基己基酯对原代培养新生雌性大鼠下丘脑神经元生殖神经内分泌相关基因表达影响

发布时间：2018-03-27 11:50

本文选题：邻苯二甲酸单乙基己基酯　切入点：下丘脑神经元　出处：《环境与健康杂志》2017年01期

【摘要】：目的探讨邻苯二甲酸单乙基己基酯(mono-2-ethylhexyl phthalate,MEHP)对新生雌性大鼠下丘脑生殖神经内分泌功能的影响。方法对出生24h内的新生雌性SD大鼠下丘脑神经元进行原代培养,分别暴露于含终浓度为0(对照)、10~(-15)、10~(-12)、10~(-9)、10~(-6)mol/L MEHP的培养基染毒24h。采用qRT-PCR法检测Gn RH、Gn RHr、Kiss1及Kiss1r基因的表达水平。结果与对照组相比,各浓度MEHP暴露组原代培养新生雌性大鼠下丘脑神经元Kiss1基因的表达水平均较低,差异有统计学意义(P0.05);而Gn RH、Gn RHr和Kiss1r基因的表达水平均无明显改变。结论 MEHP有可能通过下调新生雌性大鼠下丘脑神经元Kiss1基因表达水平来干扰生殖神经的内分泌调控功能。
[Abstract]:Objective to investigate the effect of monoethyl hexyl phthalate mono-2-ethylhexyl phthalate (MEHP) on the reproductive neuroendocrine function of hypothalamus in neonatal female rats. Methods the hypothalamic neurons of newborn female SD rats within 24 hours were cultured in primary culture. The expression levels of Gn RHN Gn RHrN Kiss1 and Kiss1r gene were detected by qRT-PCR assay. The expression level of Kiss1 gene in hypothalamic neurons of primary cultured neonatal female rats was lower in MEHP exposure group. The difference was statistically significant (P 0.05), but the expression levels of Gn RHN RHr and Kiss1r genes were not significantly changed. Conclusion MEHP may interfere with the endocrine regulation of reproductive nerve by down-regulating the expression level of Kiss1 gene in hypothalamic neurons of newborn female rats.
【作者单位】：天津医科大学公共卫生学院;
【基金】：国家自然科学基金(81273027)
【分类号】：R114

【相似文献】