液相色谱串接质谱联用快速分析肉食品中克仑特罗对映异构体

发布时间：2018-03-29 07:09

本文选题：克仑特罗对映异构体　切入点：兴奋剂　出处：《中国运动医学杂志》2015年12期

【摘要】：目的:建立液相色谱串接质谱联用法快速分析肉食品中克仑特罗对映异构体的方法,分析现有肉食品中克仑特罗对映异构体的比值情况。方法:样品经匀浆处理后,酸化提取,MCX 6cc固相萃取柱纯化,以流动相为10 mmol/L甲酸铵的甲醇为流动相,采用Astec CHIROBIOTICTM T 150 mm×2.1 mm×5μm色谱柱分离,电喷雾离子源,正离子多反应监测模式扫描分析检测,D9-克仑特罗为内标。采用该方法分析了85例含有克仑特罗肉食品中的R-(-)-克仑特罗与S-(+)-克仑特罗比值和分布情况。结果:R-(-)-克仑特罗和S-(+)-克仑特罗实现基线分离,方法的检测限为0.1μg/kg,在添加的高、中、低三种浓度的相对回收率分布在81%~111%之间。结论:该方法样品前处理简单、快速、可靠。
[Abstract]:Objective: to establish a rapid method for the determination of clenbuterol enantiomers in meat food by liquid chromatography tandem mass spectrometry (LC-MS) and to analyze the ratio of clenbuterol enantiomers in meat food. The acid extraction was purified on a MCX 6cc solid-phase extraction column. The mobile phase was methanol with 10 mmol/L ammonium formate as the mobile phase. The column was separated by Astec CHIROBIOTICTM T 150mm 脳 2.1mm 脳 5 渭 m, and the electrospray ion source was used. In this paper, the ratio and distribution of R-O-clenbuterol and S-clenbuterol in 85 meat products containing clenbuterol were analyzed with this method. Lentro and S-Clenbuterol achieve baseline separation, The detection limit of the method was 0.1 渭 g / kg. The relative recoveries of the three concentrations of high, medium and low concentrations were between 81% and 111%. Conclusion: the method is simple, rapid and reliable.
【作者单位】：国家体育总局反兴奋剂中心食品药品兴奋剂检测实验室;
【分类号】：R155.55

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