丙烯酰胺暴露对幼鼠胼胝体PLP和MBP表达的影响

发布时间：2018-04-09 14:51

本文选题：丙烯酰胺　切入点：胼胝体　出处：《神经解剖学杂志》2017年05期

【摘要】：目的:检测断乳期幼鼠胼胝体髓鞘蛋白PLP和MBP的表达,探讨丙烯酰胺(acrylamide,ACR)染毒对幼鼠胼胝体部髓鞘发育的影响。方法:断乳期幼鼠随机分为对照组(0 mg/kg)、低(18 mg/kg)和高(36 mg/kg)剂量组,每组12只,从出生后第22~42 d进行灌胃染毒。观测幼鼠步态的变化,用免疫组化方法和免疫荧光双标记法检测幼鼠胼胝体髓鞘蛋白脂蛋白(myelin PLP,PLP)和髓鞘碱性蛋白(myelin basic protein,MBP)的表达。结果:ACR染毒后幼鼠的步态评分均增加,差异有统计学意义(P0.01),免疫组织化学检测结果显示,与对照组相比较,ACR高剂量组幼鼠大脑PLP和MBP表达减少,差异有统计学意义(P0.05)。免疫荧光双标技术检测结果与免疫组织化学检测结果一致,即ACR染毒后胼胝体PLP和MBP均表达减少。结论:ACR染毒可能会通过减少PLP和MBP的表达,抑制胼胝体髓鞘的形成而影响神经系统发育。
[Abstract]:Aim: to detect the expression of myelin PLP and MBP in corpus callosum of young rats during weaning period and to investigate the effect of acrylamide acrylamide on the development of myelin in corpus callosum of young rats.Methods: young rats during weaning period were randomly divided into control group (0 mg / kg), low dose (18 mg / kg) and high dose group (36 mg / kg). 12 rats in each group were given intragastric administration from 22 to 42 days after birth.The changes of gait of young rats were observed, and the expression of myelin basic (PLP) and myelin basic protein (MBP) in corpus callosum protein (PLP) and myelin basic protein (myelin basic) in young rats were detected by immunohistochemistry and immunofluorescence double labeling method.Results the gait scores of the young rats were all increased after the treatment with 1: ACR, and the difference was statistically significant (P 0.01). The results of immunohistochemistry showed that the expression of PLP and MBP in the brain of the high dose group was lower than that of the control group, and the difference was statistically significant (P 0.05).The results of double immunofluorescence staining were consistent with those of immunohistochemistry, that is, the expression of PLP and MBP in corpus callosum decreased after ACR exposure.ConclusionACR exposure may affect the development of nervous system by reducing the expression of PLP and MBP and inhibiting the formation of corpus callosum myelin.
【作者单位】：广东药科大学基础学院人体解剖学教研室;
【基金】：广东省科技计划项目(2016A020225007)
【分类号】：R114

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