马铃薯淀粉酶法制备高麦芽糖浆工艺研究

发布时间：2018-04-17 07:13

本文选题：马铃薯淀粉 + 高麦芽糖浆　；参考：《甘肃农业大学》2012年硕士论文

【摘要】：高麦芽糖浆是以淀粉为原料，经液化、糖化和精制工艺生产的麦芽糖含量为70%-80%、葡萄糖含量低于2%的中等转化糖浆（GB/T20883-2007）。高麦芽糖浆具有甜味温和适口、风味独特、吸湿性小、抗结晶、热稳定性好、易消化吸收、清亮透明等特点，在食品、医药、轻工业等行业中用途广泛。本研究以马铃薯淀粉为原料，采用酶法制备高麦芽糖浆，利用单因素试验、Box-Behnken试验、中心复合试验以及正交试验，对制备高麦芽糖浆中的液化、糖化及精制工艺条件进行了优化研究，确定了各工序的最佳参数，并对成品进行了定性、定量分析。研究结果如下：（1）以耐高温α-淀粉酶为液化酶，DE值和透光率为衡量指标，采用单因素与Box-Behnken试验设计，，对马铃薯淀粉制备高麦芽糖浆酶法液化工艺条件进行了研究。结果表明：在液化温度96℃、液化时间15.6min、耐高温α-淀粉酶用量15.13U/g淀粉、淀粉乳浓度21.4%（w/v）、pH值为6.2以及无水CaCl_2用量为0.10%（w/w，以淀粉原料计）的条件下，所制备的马铃薯淀粉液化液DE值为10.03%。（2）以真菌α-淀粉酶、β-淀粉酶、普鲁兰酶为糖化用酶，采用单因素及中心复合试验设计，对马铃薯淀粉制备高麦芽糖浆糖化工艺条件进行了研究。结果表明：在糖化温度57.9℃、糖化时间4.74h、糖化液pH值为5.5、真菌α-淀粉酶用量2.24U/g淀粉、β-淀粉酶用量为4.76U/g淀粉、普鲁兰酶用量为12.5U/g淀粉的工艺条件下，所制备的糖化液DE值为48.11%，糖化液麦芽糖含量为65.88%、葡萄糖含量为8.81%、麦芽三糖含量为5.73%。（3）采用5种活性炭对高麦芽糖浆的脱色效果进行了试验，确定了粉末活性炭AK-220脱色效果最好。以脱色温度、脱色时间、活性炭用量及pH值为因素，透光率为指标，通过L16(4~5)正交试验设计对脱色条件进行了优化研究，结果表明：在脱色温度为65℃、脱色时间为50min、活性炭用量为3.5%（w/w，以糖液干物质计）、pH值为4.0的条件下，活性炭AK-220对麦芽糖浆脱色后的透光率为93.19%。（4）采用732阳离子交换树脂和D330阴离子交换树脂，通过阳-阴-阳树脂柱串联，对制备的麦芽糖浆进行分离纯化，结果表明：所制成品中麦芽糖含量为71.57%，较糖化后提高5.69%；葡萄糖含量为1.71%、麦芽三糖含量为1.98%，较糖化后分别降低了7.10%、4.65%。离子交换后2次脱色，麦芽糖浆透光率达到了95.52%，较1次脱色提高了1.33%。（5）以薄层色谱法对制备的高麦芽糖浆进行了定性分析，在正丁醇：乙酸：水=8:2:3（v/v）为展开剂、苯胺-二苯胺-磷酸溶液为显色剂、点样量2μL的条件下，各组分糖的分离效果好。定性结果表明：麦芽糖含量高，葡萄糖、麦芽三糖含量少。高效液相色谱定量分析结果表明：麦芽糖含量为71.57%，葡萄糖含量为1.71%，麦芽三糖含量为1.08%。（6）所制高麦芽糖浆为黏稠状透明液体，干物质含量为76.6%，pH值为5.3，DE值为44.29%，硫酸灰分含量小于0.3%，透光率为95.52%，麦芽糖含量为71.57%，葡萄糖含量为1.71%，麦芽三糖含量为1.08%，符合GB/T20883-2007标准。最终产品得率为92.15%。
[Abstract]:High maltose syrup with starch as raw material, after liquefaction, saccharification and malt sugar refining process for 70%-80%, medium glucose conversion syrup content less than 2% (GB/T20883-2007). High maltose syrup has mild sweet taste, unique flavor, moisture absorption, anti crystallization, good thermal stability, easy digestion and absorption, clear and transparent other characteristics, widely used in food, medicine, light industry and other industries.
In this study, potato starch as raw material, preparation of high maltose syrup by enzyme method, using single factor test, Box-Behnken test, central composite experiment and orthogonal test, the preparation of high maltose syrup in liquefaction, saccharification and purification process was studied, the optimum parameters of each process were determined, and the quantitative analysis of products qualitatively, the results are as follows:
(1) the thermostable alpha amylase liquefaction enzyme, DE value and light transmittance as measured by single factor test and Box-Behnken design, preparation of potato starch liquefaction process conditions of high maltose syrup by enzyme were studied. The results showed that: in the liquefaction temperature 96 C, reaction time 15.6min, thermostable alpha amylase dosage of 15.13U/g starch, starch concentration 21.4% (w/v), the pH value is 6.2 and the absolute amount of CaCl_2 was 0.10% (w/w, starch raw material) under the condition of the preparation of potato starch liquefaction DE value is 10.03%.
(2) with fungal alpha amylase, beta amylase and pullulanase as saccharifying enzyme, the composite experimental design of single factor and center of potato starch production of high maltose syrup saccharification conditions were studied. The results showed that: in the saccharification temperature of 57.9 DEG C, saccharification time 4.74h, saccharification liquid pH value was 5.5. Fungal alpha amylase dosage of 2.24U/g starch, amylase dosage of 4.76U/g starch, pullulan enzyme dosage conditions of 12.5U/g starch, the saccharification liquid synthesis of DE value is 48.11%, the content of maltose 65.88% saccharification liquid, the content of glucose was 8.81%, three malt sugar content is 5.73%.
(3) the decolorization effect of high maltose syrup with 5 kinds of activated carbon were studied, to determine the best decolorization effect of powdered activated carbon AK-220. Decolorization temperature, decolorization time, activated carbon dosage and pH value factors, the transmittance index by L16 (4~5) orthogonal design optimization study of the results showed that the decolorization decolorization conditions in the temperature is 65 DEG C, decolorization time is 50min, the dosage of activated carbon was 3.5% (w/w, by measuring the dry matter), the pH value is 4 under the condition of activated carbon AK-220 on transparent maltose syrup after decolorization rate was 93.19%.
(4) by 732 cation exchange resin and D330 anion exchange resin, by Yang - Yin - Yang resin column series, on the preparation of maltose syrup was purified, the results showed that the content of maltose by manufactured goods was 71.57%, increased by 5.69% than after saccharification; the content of glucose was 1.71%, three malt sugar content is 1.98%. Compared with after saccharification were reduced by 7.10%, 2 4.65%. after ion exchange decolorization, maltose syrup transmittance reached 95.52%, compared with 1 decolorization increased by 1.33%.
(5) by TLC on the preparation of high maltose syrup were qualitatively analyzed in n-butanol: acetic acid: water =8:2:3 (v/v) as the agent, aniline aniline - two phosphoric acid solution as the chromogenic agent, condition of sample volume of 2 L, the separation effect of components of sugar. The qualitative results showed that the content of maltose, glucose, malt sugar content of three less.
The quantitative analysis results of high performance liquid chromatography showed that the content of maltose was 71.57%, the content of glucose was 1.71%, and the content of malt three sugar was 1.08%.
(6) the preparation of high maltose syrup for viscous transparent liquid, dry matter content is 76.6%, pH = 5.3, DE = 44.29%, sulfate ash content less than 0.3%, the transmittance of 95.52%, maltose content is 71.57%, the content of glucose was 1.71%, three malt sugar content is 1.08%, according to GB/ T20883-2007 standard. The final product yield is 92.15%.

【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R151

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