化学诱导剂对寇氏隐甲藻油脂积累提高的代谢调控机理

发布时间：2018-04-18 06:29

本文选题：多不饱和脂肪酸 + C.cohnii　；参考：《天津大学》2015年硕士论文

【摘要】：多不饱和脂肪酸(Polyunsantured fatty acid,PUFAs)作为人体必需脂肪酸对人体的生理功能和身心健康具有重要的意义。尤其是ω-3 PUFAs中的二十二碳六烯酸(Docosahexaenoic acid,DHA)和二十碳五烯酸(Eicosapentaenoic acid,EPA)更是成为在生产和生活中应用广泛的高营养价值的脂肪酸。除了深海鱼油外,DHA也可以从微藻中提取。本文选取寇氏隐甲藻(Crypthecodinium cohnii)ATCC 30772这种不饱和脂肪酸含量很高的海洋微藻作为生产藻株。利用多种外源化合物(丁基羟基茴香醚、没食子酸丙脂、生物素、苹果酸钠、洛伐他汀)添加实验的方法诱导促进藻细胞的油脂积累。通过诱导实验,除了没食子酸丙脂使总脂肪酸的含量减少之外,其它的外源化合物均使隐甲藻的总脂含量增加,增加比例分别为丁基羟基茴香醚(BHA)8.8%、生物素2.8%、苹果酸钠6.43%、洛伐他汀4.16%。为了探究总脂肪酸含量提高在细胞代谢水平上产生的变化,我们选取使总脂肪酸提高比例最高的丁基羟基茴香醚添加实验作为研究对象,利用气相色谱-质谱联用技术(GC-MS)以及液相色谱-质谱联用技术(LC-MS)进行了代谢组学的分析研究。通过主成分分析(PCA)获得与油脂积累相关的某些重要代谢物的含量变化,包括二磷酸腺苷(ADP)、NADPH、5-磷酸核糖(R5P)、磷酸烯醇式丙酮酸(PEP)等重要化合物;通过热图展示了24种中心代谢物的含量变化;通过加权关联网络分析(WGCNA)分析获得8个与BHA添加相关模块以及9个关键代谢物(异麦芽糖、腺苷、对苯二酚、3-hydroxypropanoic-acid、L-异亮氨酸、L-脯氨酸、十四酸甲酯、亚油酸甲酯和丙二酸),通过这些分析结果揭示了与脂肪酸积累可能相关的代谢调控机理。
[Abstract]:Polyunsaturated fatty acids (polyunsaturated fatty acids) as essential fatty acids in human body play an important role in physiological function and physical and mental health.Especially, the 22 carbohexaenoic acid (PUFAs) and eicosapentaenoic acidic acid (EPAs) in 蠅 -3 PUFAs have been widely used in production and life as fatty acids with high nutritional value.In addition to deep-sea fish oil, DHA can also be extracted from microalgae.In this paper, marine microalgae Crypthecodinium cohnii)ATCC 30772, which has a high unsaturated fatty acid content, is selected as the algal production strain.A variety of exogenous compounds (Ding Ji hydroxy anisole, propyl gallate, biotin, sodium malate, lovastatin) were used to induce the accumulation of oil in algal cells.In addition to the decrease of total fatty acid content caused by propyl gallate, all the exogenous compounds increased the total lipid content of Cryptoid.The proportion of increase was Ding Ji hydroxyl anisole BHAA 8.8, biotin 2.8, malate sodium 6.43, lovastatin 4.16.In order to investigate the changes of total fatty acid content in cell metabolism, we selected Ding Ji hydroxyl anisole as the experimental object.The metabolomics analysis was carried out by gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS).Some important metabolites related to oil accumulation were obtained by principal component analysis (PCA), including some important compounds such as adenosine diphosphate (ADP), NADPH5 phosphate ribonucleosaccharide (R5PN), phosphoenolpyruvate (PEP) and so on.The contents of 24 central metabolites were shown by heat map, 8 modules associated with BHA addition and 9 key metabolites (isomaltose, adenosine, hydroquinone 3-hydroxypropanoic-acidic-acidic-acidic-acidicidic-L- isoleucine L-proline) were obtained by weighted correlation network analysis, and 9 key metabolites (isomaltose, adenosine, hydroquinone) were obtained.Methyl tetradecanoate, methyl linoleate and malonate revealed the metabolic regulation mechanism related to fatty acid accumulation.
【学位授予单位】：天津大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R151.41

【参考文献】