稀土氧化钕暴露对大鼠肺泡巨噬细胞毒性及炎性因子分泌的影响

发布时间：2018-04-24 10:31

本文选题：稀土氧化钕 + 大鼠肺泡巨噬细胞　；参考：《环境与健康杂志》2015年10期

【摘要】：目的研究稀土氧化钕暴露对大鼠肺泡巨噬细胞(NR8383)的毒性及其炎性因子分泌的影响。方法将处于对数生长期的NR8383细胞,暴露于终浓度分别为0(对照,PBS)、12.5、25、50、100、200μg/ml的氧化钕混悬液12、24、48 h后,采用MTT法测定NR8383细胞的抑制率;终浓度为0(对照,PBS)、25、50、100μg/ml的氧化钕混悬液24 h后,采用酶标法测定细胞培养液上清中肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)及白介素-8(IL-8)的浓度。结果与对照组比较,各浓度氧化钕暴露不同时间后NR8383细胞的抑制率均较高,各浓度氧化钕暴露NR8383细胞上清中TNF-α、IL-1β、IL-8的浓度均较高,差异有统计学意义(P0.05);且随着氧化钕暴露浓度的升高,NR8383细胞的抑制率及上清中TNF-α、IL-1β、IL-8的浓度均呈上升趋势。氧化钕暴露NR8383细胞的抑制率与上清中TNF-α、IL-1β、IL-8的浓度均呈正相关(P0.01);且NR8383细胞上清中TNF-α、IL-1β、IL-8浓度两两之间均呈正相关(P0.01)。结论氧化钕刺激可引起NR8383细胞分泌炎性因子增加。
[Abstract]:Objective to study the toxicity of neodymium oxide exposure to alveolar macrophages (NR8383) and the secretion of inflammatory cytokines in rats. Methods NR8383 cells in logarithmic growth phase were exposed to neodymium oxide suspension with final concentration of 0 (control group, 12.5U 2550100200 渭 g/ml) for 48 h, then the inhibition rate of NR8383 cells was measured by MTT assay, and the final concentration was 0 (control group) 2550100 渭 g/ml neodymium oxide suspension for 24 h. The concentrations of tumor necrosis factor- 伪 (TNF- 伪), interleukin-1 尾 (IL-1 尾) and interleukin-8 (IL-8) in the supernatant of cell culture medium were determined by enzyme labeling method. Results compared with the control group, the inhibition rate of NR8383 cells was higher at different concentrations of neodymium oxide, and the concentration of TNF- 伪 -IL-1 尾 IL-8 in the supernatant of NR8383 cells exposed to neodymium oxide at different concentrations was higher than that in the control group. The inhibitory rate of NR8383 cells and the concentration of TNF- 伪 -IL-1 尾 -IL-8 in the supernatant of NR8383 cells increased with the increase of neodymium oxide exposure concentration. The inhibition rate of neodymium oxide exposure to NR8383 cells was positively correlated with the concentration of IL-8 in the supernatant of TNF- 伪 and IL-1 尾, and the concentration of IL-8 in the supernatant of NR8383 cells was positively correlated with the concentration of TNF- 伪, IL-1 尾 and IL-8 in the supernatant of NR8383 cells. Conclusion the stimulation of neodymium oxide can increase the secretion of inflammatory factors in NR8383 cells.
【作者单位】：包头医学院公共卫生学院;内蒙古巴彦淖尔市疾病预防控制中心;
【基金】：国家自然科学基金(81260426)
【分类号】：R114

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