环磷酸腺苷信号对矽肺纤维化形成的影响

发布时间：2018-04-26 10:12

  本文选题：矽肺病 + 肌成纤维细胞　； 参考：《中国现代医学杂志》2017年06期

【摘要】：目的观察环磷酸腺苷(cAMP)信号在大鼠矽肺纤维化发生、发展过程中的变化及其对矽肺纤维化形成的影响。方法采用HOPE-MED8050动式染尘控制系统复制大鼠矽肺模型,雄性Wistar大鼠随机分为6组,每组10只,分别染尘0、2、4、8、12和16周。采用Masson三色染色观察肺组织形态,采用免疫组织化学法染色和Western blot检测α-平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原(CollagenⅠ)、纤连蛋白(Fn)、激动型G蛋白α(Gαs)、抑制型G蛋白α(Gαi2、Gαi3)及cA MP的表达。结果 Masson三色染色显示,矽肺模型4周组细胞性结节区域可见蓝紫色胶原沉积,并随模型制备时间的延长纤维化病变区域胶原沉积逐渐增加。免疫组织化学法染色显示,对照组α-SMA阳性表达部位在气管及血管平滑肌;在矽肺模型16周组,α-SMA表达于结节周边及间质纤维化病变区域。α-SMA、CollagenⅠ、Fn及Gαi2、Gαi3蛋白表达在矽肺模型4、8、12和16周较对照组逐渐增多。在矽肺模型8周组Gαs蛋白表达及cAMP含量较对照组下降,至染尘16周达最低。结论 cAMP的表达在矽肺发生、发展过程中呈下降趋势,cAMP信号参与大鼠矽肺纤维化过程。
[Abstract]:Aim to observe the changes of cyclic adenosine monophosphate (camp) signal in the pathogenesis and development of silicosis in rats and its effect on the formation of silicosis. Methods the silicosis model of rats was induced by HOPE-MED8050 dynamic dust control system. Male Wistar rats were randomly divided into 6 groups with 10 rats in each group for 12 and 16 weeks respectively. The expression of 伪 -smooth muscle actin (伪 -SMAA), fibronectin (FN), activated G protein 伪 G 伪 sn, inhibitory G protein 伪 G 伪 I 2G 伪 I 3 and camp were detected by immunohistochemical staining and Western blot staining. Results Masson trichrome staining showed that blue-purple collagen deposition could be seen in the cellular nodules of the silicosis model for 4 weeks, and the collagen deposition increased gradually with the prolongation of the time of the establishment of the model. Immunohistochemical staining showed that the positive expression of 伪 -SMA in the control group was located in trachea and vascular smooth muscle. In 16 weeks group, 伪 -SMA was expressed around the nodules and in the interstitial fibrosis lesions. The expression of 伪 -SMA-Collagen 鈪，

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